TCF-3/E2A [p Thr355] Antibody

Product Discontinued

TCF-3/E2A [p Thr355] Antibody Summary

• Immunogen: A synthetic phospho-peptide, which represented a portion of human E2A immunoglobulin enhancer binder factors E12/E47 around threonine 355 (LocusLink ID 6929).
• Modification: p Thr355
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Application Notes: Western Blot, where a band should be seen at ~70-80 kDa. *The investigator should determine the optimal working dilution for a specific application.
• Control:

  TCF-3/E2A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TCF-3/E2A [p Thr355] Antibody

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

E2A proteins are ubiquitously expressed. They are important transcription factors downstream of the T-cell receptor (TCR) signal that regulate multiple stages of thymocyte development and suppress T-cell lymphoma. Deficiencies in E2A promote age-dependent autoimmune diseases, such as T lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF-3/E2A Antibody (NB100-394)(3)

Publications using NB100-394

• King AM, Van der Put E, Blomberg BB et al. Accelerated Notch-dependent degradation of E47 proteins in aged B cell precursors is associated with increased ERK MAPK activation. J Immunol 2007-03-01 [PMID: 17339447]
• Cole F, Zhang W, Geyra A et al. Positive regulation of myogenic bHLH factors and skeletal muscle development by the cell surface receptor CDO. Dev Cell 2004-12-01 [PMID: 15572127]
• Nie L, Xu M, Vladimirova A et al. Notch-induced E2A ubiquitination and degradation are controlled by MAP kinase activities. EMBO J 2003-11-01 [PMID: 14592976]

Bioinformatics

• Gene Symbol: TCF3
• Entrez:
  • 6929(Human)