Recombinant Human TCEB1 His Protein

SDS-Page: Recombinant Human TCEB1 Protein [NBP1-78862]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human TCEB1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-112 of Human TCEB1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ELOC
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 14.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TCEB1 His Protein

• ELOC
• elongin 15 kDa subunit
• elongin-C
• RNA polymerase II transcription factor SIII subuni
• RNA polymerase II transcription factor SIII subunit C
• SIII p15
• SIII
• TCEB1
• transcription elongation factor B (SIII), polypept
• transcription elongation factor B (SIII), polypeptide 1 (15kD, elongin C)
• transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• transcription elongation factor B polypeptide 1
• transcription elongation factor B subunit 1
• transcription elongation factor B, polypeptide 1

Background

TCEB1 (elongin C) is a subunit of elongin, which is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template encoded arresting sites. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau(VHL) tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant human TCEB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ELOC