TATA binding protein TBP Antibody (1TBP18)

Product Discontinued

TATA binding protein TBP Antibody (1TBP18) Summary

• Immunogen: Synthetic peptide corresponding to the N-terminus of the human TATA-binding protein.
• Epitope: Within amino acid residues 1-20 of human, mouse and rat TBP.The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.
• Localization: Nuclear loading control
• Specificity: This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Gel Super Shift Assays 1:1-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2 ug/ml
• Control:

  TATA binding protein TBP Overexpression Lysate

Reactivity Notes

It does not react with TBP from Drosophila, Yeast, Silk Worm, or Xenopus. Other species not investigated. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 1.7 mg/ml
• Purity: Protein G purified

Notes

This antibody may be used in Western blot and electrophoretic mobility (gel shift) assays to detect mammalian TBP. It may also be used to immunoprecipitate a truncated TBP molecule containing only the N-terminal domain.

Alternate Names for TATA binding protein TBP Antibody (1TBP18)

• GTF2D
• GTF2D1
• HDL4
• MGC126054
• MGC126055
• SCA17
• TATA box binding protein
• TATA sequence-binding protein
• TATA-binding factor
• TATA-box binding protein N-terminal domain
• TATA-box factor
• TATA-box-binding protein
• TF2D
• TFIIDMGC117320
• Transcription initiation factor TFIID TBP subunit

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein TBP and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.TPB is also known to interact with HIV1 Tat protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TATA binding protein TBP Antibody (NB500-700)(9)

Publications using NB500-700

• Matsumura Y, Ito R, Yajima A et al. Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis Nat Commun 2021-12-03 [PMID: 34857762]
• Inagaki T, Iwasaki S, Matsumura Y et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J. Biol. Chem. 2014-12-22 [PMID: 25533466] (Mouse)
• Lalevee S, Lapaire O, Buhler M. miR455 is linked to hypoxia signaling and is deregulated in preeclampsia. Cell Death Dis. 2014-09-04 [PMID: 25188518]
• Sheldon Kathryn E, Shandilya Harish, Kepka-Lenhart Diane et al. Shaping the murine macrophage phenotype: IL-4 and cyclic AMP synergistically activate the arginase I promoter. J Immunol 2013-01-01 [PMID: 23913966] (WB, WB, Mouse)
• An CI, Ganio E, Hagiwara N. Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells. Skelet Muscle 2013-05-10 [PMID: 23663701] (WB, Mouse)
• Chuang CH, Wallace MD, Abratte C et al. Incremental genetic perturbations to MCM2-7 expression and subcellular distribution reveal exquisite sensitivity of mice to DNA replication stress. PLoS Genet. 2010-09-09 [PMID: 20838603] (WB, Mouse)
• Wakabayashi K, Okamura M, Tsutsumi S et al. The peroxisome proliferator-activated receptor gamma/retinoid X receptor alpha heterodimer targets the histone modification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop. Mol Cell Biol. 2009-07-01 [PMID: 19414603] (WB, Mouse)
• Okamura M, Kudo H, Wakabayashi K-i et al. COUP-TFII acts downstream of Wnt/{beta}-catenin signal to silence PPAR{gamma} gene expression repress adipogenesis. PNAS;106(14):5819-5824. 2009-01-01 [PMID: 19307559]
• Reagan-Shaw, S et al. RNA Interference-Mediated Depletion of Phosphoinositide 3-Kinase Activates Forkhead Box Class O Transcription Factors Induces Cell Cycle Arrest Apoptosis in Breast Carcinoma Cells. Cancer Res; 66: (2), 2006. 2006-01-01 [PMID: 16424042]

Reviews for TATA binding protein TBP Antibody (NB500-700) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Adam Causgrove ELISA Mouse 12/17/2010

Summary

• Application: ELISA
• Sample Tested: Mouse
• Species: Mouse

reviewed by: DANISH SAYED WB Rat 09/23/2010

Summary

• Application: Western Blot
• Sample Tested: Cultured Neonatal Myocytes, Sample Amount: 15-20ug of protein
• Species: Rat

Bioinformatics

• Gene Symbol: TBP
• Entrez:
  • 6908(Human)
  • 21374(Mouse)
  • 117526(Rat)
• Uniprot:
  • P20226(Human)