TATA binding protein TBP Overexpression Lysate

Images

 
Western Blot: TATA binding protein TBP Overexpression Lysate (Adult Normal) [NBL1-16741] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TATA binding protein TBP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TATA binding protein TBP Overexpression Lysate Summary

Description

TATA binding protein TBP Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210727

Accession#: NM_003194

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
TBP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TATA binding protein TBP Overexpression Lysate

  • GTF2D
  • GTF2D1
  • HDL4
  • MGC126054
  • MGC126055
  • SCA17
  • TATA box binding protein
  • TATA sequence-binding protein
  • TATA-binding factor
  • TATA-box binding protein N-terminal domain
  • TATA-box factor
  • TATA-box-binding protein
  • TF2D
  • TFIIDMGC117320
  • Transcription initiation factor TFIID TBP subunit

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for TATA binding protein TBP Lysate (NBL1-16741) (0)

There are no publications for TATA binding protein TBP Lysate (NBL1-16741).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TATA binding protein TBP Lysate (NBL1-16741) (0)

There are no reviews for TATA binding protein TBP Lysate (NBL1-16741). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TATA binding protein TBP Lysate (NBL1-16741) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TATA binding protein TBP Products

Research Areas for TATA binding protein TBP Lysate (NBL1-16741)

Find related products by research area.

Blogs on TATA binding protein TBP

There are no specific blogs for TATA binding protein TBP, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TATA binding protein TBP Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol TBP