Tankyrase 1 Antibody

Western Blot: Tankyrase 1 Antibody [NBP1-36994] - HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4) and probed with EB02508 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4 degrees C. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tankyrase 1 Antibody Summary

• Description: Novus Biologicals Goat Tankyrase 1 Antibody (NBP1-36994) is a polyclonal antibody validated for use in WB. Anti-Tankyrase 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RECOMBINANT corresponding to N-Terminus according to NP_003738.2.
• Epitope: N Terminal Region
• Specificity: Fusion protein containing 120aa amino acids from N Terminal region (according to NP_003738.2)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNKS
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing TANK1 a band of approx. 142 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 142 kDa band according to NP_003738.2.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Tankyrase 1 Antibody

• EC 2.4.2.30
• PARP5A
• PARP-5a
• PARP5APARPL
• PARPL
• pART5
• Poly [ADP-ribose] polymerase 5A
• TANK1
• Tankyrase 1
• Tankyrase I
• tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
• tankyrase-1
• TIN1TANK1
• TINF1
• TINF1TNKS-1
• TNKS
• TNKS1TRF1-interacting ankyrin-related ADP-ribose polymerase

Background

Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase co-localizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tankyrase 1 Antibody (NBP1-36994)(1)

Publication using NBP1-36994

• Seimiya H, Muramatsu Y, Ohishi T, Tsuruo T. Tankyrase 1 as a target for telomere-directed molecular cancer therapeutics. Cancer Cell 2005-01-01 [PMID: 15652747]

Bioinformatics

• Gene Symbol: TNKS
• Entrez:
  • 8658(Human)
• Uniprot:
  • NP_003738.2(Human)