Tankyrase 1 Antibody

Images

 
Western Blot: Tankyrase 1 Antibody [NB100-186] - Detection of tankyrase in DU145 whole cell lysates (50 ug) using NB 100-186. ECL detection - 30 seconds. Lane 1 - 1:500 primary dilution Lane 2 - 1:2,000 primary dilution
Immunocytochemistry/ Immunofluorescence: Tankyrase 1 Antibody [NB100-186] - Tankyrase antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Tankyrase 1 Antibody Summary

Immunogen
A synthetic peptide made to a region of human Tankyrase within residues 250-350 [UniProt# O95271]. The actual peptide sequence is proprietary.
Localization
Cytoplasmic. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of Tankyrase is found in the nucleus, associated with TRF1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNKS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:250-1:500
Application Notes
This Tankyrase antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where it recognizes an endogenous band at ~150 kDa in whole cell extract and a band at ~180 kDa in baculovirus-derived his-tagged protein.
Positive Control
HeLa Lysate (NB800-PC1)
Control Peptide
Tankyrase 1 Peptide (NB100-186PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tankyrase 1 Antibody

  • EC 2.4.2.30
  • PARP5A
  • PARP-5a
  • PARP5APARPL
  • PARPL
  • pART5
  • Poly [ADP-ribose] polymerase 5A
  • TANK1
  • Tankyrase 1
  • Tankyrase I
  • tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
  • tankyrase-1
  • TIN1TANK1
  • TINF1
  • TINF1TNKS-1
  • TNKS
  • TNKS1TRF1-interacting ankyrin-related ADP-ribose polymerase

Background

The ~142 kDa Tankyrase protein is ubiquitously expressed in mammalian cells and is localized to human telomeres. Tankyrase has homology to ankyrins and to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase binds to TRF1 (telomeric repeat binding factor-1), which negatively regulates the maintenance of telomere length.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Tankyrase 1 Antibody (NB100-186): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tankyrase 1 Antibody (NB100-186) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-186AF405
Alexa Fluor 488 Labeled NB100-186AF488
Alexa Fluor 647 Labeled NB100-186AF647
Alexa Fluor 700 Labeled NB100-186AF700
Biotin Labeled NB100-186B
DyLight 405 Labeled NB100-186V
DyLight 488 Labeled NB100-186G
DyLight 550 Labeled NB100-186R
DyLight 650 Labeled NB100-186C
FITC Labeled NB100-186F
HRP Labeled NB100-186H

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Bioinformatics

Gene Symbol TNKS
Entrez