Talin1 Antibody (8D4)

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Immunocytochemistry/ Immunofluorescence: Talin1 Antibody (8D4) [NB600-1029] - Immunofluorescent staining of rat embryo fibroblasts using Monoclonal Anti-Talin, (clone no. 8d4, Cat. No. T3287), showing reactivity with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, ChSpecies Glossary
Applications WB, ICC/IF, IP
Clone
8D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Talin1 Antibody (8D4) Summary

Immunogen
Cytoskeletal protein talin, purified from chicken gizzard.
Localization
Cell Membrane and Intracllular
Specificity
Monoclonal anti-Talin recognizes an epitope located on the intact talin molecule (225 kDa) and the 190 kDa fragment but not on the 47 kDa fragment obtained by protease cleavage of chicken gizzard talin, when used in immunoblotting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TLN1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
immunofluorescence and Western Blot: In WB expected bands at 225 kDa and 190 kDa. The antibody also stains a slightly higher molecular weight talin (235 kDa) of mammalian platelets.The antibody stains focal adhesions, the membrane ruffles, and ventral streaks in cultured fibroblasts of mammalian (human, rat), avian (chicken) and amphibian (Xenopus), but not fish, in indirect immunofluorescence. NB120-11188 may also be used microinjection and transfection studies. Optimal dilutions and concentrations should be determined by the end user.
Theoretical MW
230 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Frog. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Raw ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Talin1 Antibody (8D4)

  • ILWEQ
  • KIAA1027
  • talin 1
  • Talin1
  • talin-1
  • TLN
  • TLN1

Background

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Talin1 Antibody (NB600-1029). (Showing 1 - 1 of 1 FAQ).

  1. I bought Talin antibody (8d4) for western blot and it looks like this product is acites, not purified antibody. What kind of blocking method do you recommend, BSA, or milk, or something else?
    • This antibody is unpurified ascites. We recommend using this antibody in western blot with 5% nonfat dry milk in TBST (0.1% Tween-20). We will use this buffer for blocking (1-2 hours room temperature) and for the antibody diluent.

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Bioinformatics

Gene Symbol TLN1
Entrez
Uniprot