TAK1 Antibody (2E10) - Azide and BSA Free

Western Blot: TAK1 Antibody (2E10) [H00006885-M02] - Analysis of MAP3K7 expression in transfected 293T cell line by MAP3K7 monoclonal antibody (M02), clone 2E10.Lane 1: MAP3K7 transfected lysate(63.8 KDa).Lane 2: Non-transfected lysate.

Western Blot: TAK1 Antibody (2E10) [H00006885-M02] - Analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi ( Cat # H00006885-R02V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10 (Cat # H00006885-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA: TAK1 Antibody (2E10.) [H00006885-M02] - TAK1 Antibody (2E10) [H00006885-M02] - Detection limit for recombinant GST tagged MAP3K7 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 2E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TAK1 Antibody (2E10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAP3K7 (AAH17715, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIAATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS
• Localization: Cytoplasmic
• Specificity: MAP3K7 - mitogen-activated protein kinase kinase kinase 7
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP3K7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein on ELISA. It has been used for RNAi Validation and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TAK1 Antibody (2E10) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.25
• MAP3K7
• MEKK7
• mitogen-activated protein kinase kinase kinase 7
• TAK1
• TAK1/MAP3K7
• TAK1TGF1a
• TGF1a
• TGF-beta activated kinase 1
• TGF-beta-activated kinase 1
• Transforming growth factor-beta-activated kinase 1

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAK1 Antibody (H00006885-M02)(1)

Publication using H00006885-M02

• Rui Y, Su J, Wang H et al. Disruption of MDA5 mediated innate immune responses by the 3C proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68. J. Virol. 2017-04-19 [PMID: 28424289]

Bioinformatics

• Gene Symbol: MAP3K7
• Entrez:
  • 6885(Human)
• OMIM:
  • 602614(Human)
• Uniprot:
  • AAH17715(Human)