TACI/TNFRSF13B/CVID Antibody (166010) [Allophycocyanin]

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse TACI/TNFRSF13B APC-conjugated Monoclonal Antibody (Catalog # FAB1041A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 166010
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

TACI/TNFRSF13B/CVID Antibody (166010) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TACI/TNFRSF13B
  Phe5-Thr129
  Accession # Q9ET35
• Specificity: Detects mouse TACI/TNFRSF13B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) TACI/TNFRSF13B or rhBCMA is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFRSF13B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TACI/TNFRSF13B/CVID Antibody (166010) [Allophycocyanin]

• CD267 antigen
• CD267
• CVID
• CVID2
• FLJ39942
• MGC133214
• TACI
• TACIMGC39952
• TNFRSF13B
• TNFRSF14B
• Transmembrane activator and CAML interactor
• tumor necrosis factor receptor 13B
• tumor necrosis factor receptor superfamily member 13B
• tumor necrosis factor receptor superfamily, member 13B

Background

TACI, Transmembrane Activator and CAML-Interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell Maturation Factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

1. Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
2. von Bulow, G.U. et al. (1997) Science 278:138.
3. Gross, J.A. et al. (2000) Nature 404:995.
4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
5. Yan, M. et al. (2000) Nature Immunol. 1:37.
6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACI/TNFRSF13B/CVID Antibody (FAB1041A)(1)

Publication using FAB1041A

• Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity. J Immunol, 2014-12-10;194(2):542-52. 12/10/2014 [PMID: 25505277]

Bioinformatics

• Gene Symbol: TNFRSF13B
• Uniprot:
  • Q9ET35()