TACE/ADAM17 Antibody (111633) [Unconjugated]

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

TACE/ADAM17 knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the TACE/ADAM17 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Effects of the engineered S197P mutation on CD16a shedding in NK cells.NK92 cells transduced with empty vector (vector only), CD16a, or CD16a/S197P were treated without (Unstim.) or with PMA (100ng/ml) for 30 minutes at 37°C (A), with IL-12 and IL-18 (100ng/ml and 400ng/ml, respectively) for 24 hours at 37°C (B), or with Raji cells and rituximab for 60 min at 37°C (C). Cell surface levels of CD16a were determined by flow cytometry. Isotype-matched negative control antibody staining is indicated by a dotted line. (D) Parent NK92 cells and transduced cells expressing CD16a or CD16a/S197P were treated with Raji cells and rituximab in the presence or absence of the ADAM17 inhibitor BMS566394 (5μM) for 60 min at 37°C. Soluble CD16a levels were determined by ELISA. Each treatment condition was repeated 3 times and the data are representative of 3 independent experiments. Bar graphs show mean ± SD. Statistical significance is indicated as ***P<0.001. (E) NK92 cells expressing CD16a or CD16a/S197P were stained with the anti-ADAM17 mAbs M220, 623, 633, or an isotype-matched negative control antibody, as indicated. (F) CD56+CD45+ NK cells derived from mock-transduced iPSCs (left panel) or iPSCs expressing recombinant CD16a or CD16a/S197P (right panels) were incubated with or without K562 target cells for 4 hours at 37°C. For all histogram plots, the x-axis = Log 10 fluorescence, the y-axis = cell number, and the data are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121788), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Flow, IP, CyTOF-ready, KO
• Clone: 111633
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH
• Suitable Sample Type: 23038

TACE/ADAM17 Antibody (111633) [Unconjugated] Summary

• Immunogen: Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
  Pro18-Asn671
  Accession # P78536
• Specificity: Detects the ectodomain of human TACE/ADAM17 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM17
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TACE/ADAM17 Antibody (111633) [Unconjugated]

• ADAM 17
• ADAM metallopeptidase domain 17
• ADAM metallopeptidase domain 18
• ADAM17
• ADAM18
• CD156b antigen
• CD156b
• CSVP
• disintegrin and metalloproteinase domain-containing protein 17
• EC 3.4.24.86
• MGC71942
• Snake venom-like protease
• TACE
• TACEcSVP
• TNF-alpha convertase
• TNF-alpha converting enzyme
• TNF-alpha-converting enzyme
• tumor necrosis factor, alpha, converting enzyme

Background

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha , TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha , Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACE/ADAM17 Antibody (MAB9301)(18)

Publications using MAB9301

• Chen, HW;Zaruba, M;Dawood, A;Düsterhöft, S;Lamp, B;Ruemenapf, T;Riedel, C; Modulation of ADAM17 Levels by Pestiviruses Is Species-Specific Viruses 2024-10-02 [PMID: 39459898] (Flow Cytometry, Human)
• Vu Thuy Khanh Le-Trilling, Fabienne Maa beta en, Benjamin Katschinski, Hartmut Hengel, Mirko Trilling Deletion of the non-adjacent genes UL148 and UL148D impairs human cytomegalovirus-mediated TNF receptor 2 surface upregulation Frontiers in Immunology 2023-08-03 [PMID: 37600801]
• Rubina, A;Patel, M;Nightingale, K;Potts, M;Fielding, CA;Kollnberger, S;Lau, B;Ladell, K;Miners, KL;Nichols, J;Nobre, L;Roberts, D;Trinca, TM;Twohig, JP;Vlahava, VM;Davison, AJ;Price, DA;Tomasec, P;Wilkinson, GWG;Weekes, MP;Stanton, RJ;Wang, ECY; ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways Proceedings of the National Academy of Sciences of the United States of America 2023-08-15 [PMID: 37561786] (Flow Cytometry, Human)
• M Kwak, KM Southard, WR Kim, A Lin, NH Kim, R Gopalappa, HJ Lee, M An, SH Choi, Y Jung, K Noh, J Farlow, A Georgakopo, NK Robakis, MK Kang, ML Kutys, D Seo, HH Kim, YH Kim, J Cheon, ZJ Gartner, YW Jun Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling Nature Cell Biology, 2022-12-01;24(12):1739-1753. 2022-12-01 [PMID: 36456828] (ICC, Human)
• Somasundaram A, Cillo A, Lampenfeld C, Workman C, Kunning S, Oliveri L, Velez M, Joyce S, Calderon M, Dadey R, Rajasundaram D, Normolle D, Watkins S, Herman J, Kirkwood J, Lipson E, Ferris R, Bruno T, Vignali D Systemic Immune Dysfunction in Cancer Patients Driven by IL6 Induction of LAG3 in Peripheral CD8+ T Cells. Cancer Immunol Res, 2022-07-01;10(7):885-899. 2022-07-01 [PMID: 35587532] (Flow Cytometry, Human)
• AA Giese, A Babendreye, P Krappen, A Gross, P Strnad, S Düsterhöft, A Ludwig Inflammatory activation of surface molecule shedding by upregulation of the pseudoprotease iRhom2 in colon epithelial cells Scientific Reports, 2021-12-20;11(1):24230. 2021-12-20 [PMID: 34930929] (Flow Cytometry, Western Blot, Human)
• EJ Pomeroy, JT Hunzeker, MG Kluesner, WS Lahr, BA Smeester, MR Crosby, CL Lonetree, K Yamamoto, L Bendzick, JS Miller, MA Geller, B Walcheck, M Felices, BR Webber, TK Starr, BS Moriarity A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer Immunotherapy Mol. Ther., 2019-10-15;0(0):. 2019-10-15 [PMID: 31704085] (Flow Cytometry, Human)
• AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017-05-09;8(19):31959-31976. 2017-05-09 [PMID: 28404876] (Flow Cytometry, Human)
• PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17 J. Biol. Chem, 2017-02-23;0(0):. 2017-02-23 [PMID: 28232483] (Flow Cytometry, Human)
• Sarah Louise Dombernowsky, Jacob Samsøe-Petersen, Camilla Hansson Petersen, Rachael Instrell, Anne-Mette Bornhardt Hedegaard, Laurel Thomas et al. The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17 Nature Communications 2015-06-25 [PMID: 26108729] (Proximity Ligation Assay, Human)
• Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol, 2015-06-12;195(2):736-48. 2015-06-12 [PMID: 26071561] (Flow Cytometry, Human)
• Jing Y, Ni Z, Wu J, Higgins L, Markowski T, Kaufman D, Walcheck B Identification of an ADAM17 cleavage region in human CD16 (FcgammaRIII) and the engineering of a non-cleavable version of the receptor in NK cells. PLoS ONE, 2015-03-27;10(3):e0121788. 2015-03-27 [PMID: 25816339] (Flow Cytometry, Human)
• Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids? Cardiovasc Res, 2012-09-20;97(1):125-33. 2012-09-20 [PMID: 22997156] (Flow Cytometry, Human)
• Breshears L, Schlievert P, Peterson M A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1). J Biol Chem, 2012-07-25;287(39):32578-87. 2012-07-25 [PMID: 22833676] (Flow Cytometry, Human)
• Gutierrez-Lopez MD, Gilsanz A, Yáñez-Mó M, Ovalle S, Lafuente EM, Dominguez C, Monk PN, Gonzalez-Alvaro I, Sanchez-Madrid F, Cabanas C The sheddase activity of ADAM17/TACE is regulated by the tetraspanin CD9. Cell. Mol. Life Sci., 2011-03-02;68(19):3275-92. 2011-03-02 [PMID: 21365281] (ICC, Human)
• Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Exp. Hematol., 2009-05-03;37(7):849-56. 2009-05-03 [PMID: 19410025] (ICC, Human)
• Hurst LA, Bunning RA, Couraud PO, Romero IA, Weksler BB, Sharrack B, Woodroofe MN Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. J. Neuroimmunol., 2009-03-25;210(1):108-12. 2009-03-25 [PMID: 19324423] (ICC, Human)
• Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases. Blood, 2009-03-03;113(19):4799-809. 2009-03-03 [PMID: 19258599] (Flow Cytometry, Human)

Review for TACE/ADAM17 Antibody (MAB9301) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Immunofluorescence in fresh tissue Mouse 04/30/2019

Summary

• Application: Other
• Sample Tested: Mesenteric artery-whole
• Species: Mouse
• Lot: G-14

Bioinformatics

• Gene Symbol: ADAM17
• Uniprot:
  • P78536()