TACC3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TACC3 Antibody [NBP1-84810] - Staining of human cell line U-251 MG shows localization to cytosol & microtubule organizing center. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TACC3 Antibody [NBP1-84810] - Staining in human testis and endometrium tissues using anti-TACC3 antibody. Corresponding TACC3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: TACC3 Antibody [NBP1-84810] - Staining of human endometrium shows low expression as expected.

Immunohistochemistry-Paraffin: TACC3 Antibody [NBP1-84810] - Staining of human testis shows high expression.

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-TACC3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TACC3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TACC3 Antibody - BSA Free (NBP1-84810) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGCPAGVGTPVPADGTQTL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TACC3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TACC3 Protein (NBP1-84810PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TACC3 Antibody - BSA Free

• ERIC-1
• ERIC1ERIC-1
• MGC117382
• MGC133242
• TACC3
• transforming acidic coiled-coil-containing protein 3
• transforming, acidic coiled-coil containing protein 3

Background

The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. Expression of this gene is up-regulated in some cancer cell lines, and in embryonic day 15 in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TACC3