TACC3 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TACC3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TACC3 at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TACC3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TACC3
  Glu689-Ile838
  Accession # Q9Y6A5
• Specificity: Detects human TACC3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACC3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TACC3 Antibody [Unconjugated]

• ERIC-1
• ERIC1ERIC-1
• MGC117382
• MGC133242
• TACC3
• transforming acidic coiled-coil-containing protein 3
• transforming, acidic coiled-coil containing protein 3

Background

Transforming acidic coiled-coil containing protein 3 (TACC3; also know as ERIC-1) is a 92‑140 kDa member of the TACC family of transcriptional regulatory proteins. It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium. When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle. It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription. Human TACC3 is 838 amino acids (aa) in length and contains one poly-Serine region (aa 155‑160) and a TACC coiled-coil domain (aa 637‑837). There is one potential splice variant that shows a Lys substitution for aa 102‑462. Over amino acids 689‑838, human TACC3 shares 79% aa identity with mouse TACC3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACC3 Antibody (AF5720)(7)

Publications using AF5720

• Pace, A;Scirocchi, F;Napoletano, C;Zizzari, IG;Po, A;Megiorni, F;Asquino, A;Pontecorvi, P;Rahimi, H;Marchese, C;Ferretti, E;Nuti, M;Rughetti, A; Targeting FGFRs by pemigatinib induces G1 phase cell cycle arrest, cellular stress and upregulation of tumor suppressor microRNAs Journal of translational medicine 2023-09-15 [PMID: 37715207] (Western Blot, Human)
• WS Yuen, QH Zhang, A Bourdais, D Adhikari, G Halet, J Carroll Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes Open Biology, 2023-03-08;13(3):220326. 3/8/2023 [PMID: 36883283]
• Ana G, Bernardo O, Marco N et al. Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells. Curr Biol. 8/27/2022 [PMID: 36057259]
• Ana G, Bernardo O, Marco N et al. Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells. Curr Biol. 2022-08-27 [PMID: 36057259]
• Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle Defining endogenous TACC3–chTOG–clathrin–GTSE1 interactions at the mitotic spindle using induced relocalization Journal of Cell Science 2021-02-01 [PMID: 33380489]
• So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 6/28/2019 [PMID: 31249032]
• C Daly, C Castanaro, W Zhang, Q Zhang, Y Wei, M Ni et al. FGFR3-TACC3 fusion proteins act as naturally occurring drivers of tumor resistance by functionally substituting for EGFR/ERK signaling Oncogene 2017-01-01 [PMID: 27345413] (Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: TACC3
• Uniprot:
  • Q9Y6A5(Human)