TACC3 Antibody (OTI6F1) - Azide and BSA Free

Western Blot: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3.

Immunocytochemistry/Immunofluorescence: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 .

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Human pancreas tissue using anti-TACC3 mouse monoclonal antibody.

Flow Cytometry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TACC3 antibody, and then analyzed by flow cytometry.

Western Blot: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TACC3 mouse monoclonal antibody.

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Human colon tissue using anti-TACC3 mouse monoclonal antibody.

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Human Kidney tissue using anti-TACC3 mouse monoclonal antibody.

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Human liver tissue using anti-TACC3 mouse monoclonal antibody.

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Human lung tissue using anti-TACC3 mouse monoclonal antibody.

Immunohistochemistry: TACC3 Antibody (OTI6F1) - Azide and BSA Free [NBP2-74436] - Staining of paraffin-embedded Human prostate tissue using anti-TACC3 mouse monoclonal antibody.

• Reactivity: Hu, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI6F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TACC3 Antibody (OTI6F1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TACC3 Antibody (OTI6F1) - Azide and BSA Free (NBP2-02619) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TACC3(NP_006333) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
• Theoretical MW: 90.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TACC3 Antibody (OTI6F1) - Azide and BSA Free

• ERIC-1
• ERIC1ERIC-1
• MGC117382
• MGC133242
• TACC3
• transforming acidic coiled-coil-containing protein 3
• transforming, acidic coiled-coil containing protein 3

Background

The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. Expression of this gene is up-regulated in some cancer cell lines, and in embryonic day 15 in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TACC3