T Plastin Antibody (5B9) - Azide and BSA Free

Western Blot: T Plastin Antibody (5B9) [H00005358-M01] - Analysis of PLS3 expression in transfected 293T cell line by PLS3 monoclonal antibody (M01), clone 5B9.Lane 1: PLS3 transfected lysate (Predicted MW: 70.8 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: T Plastin Antibody (5B9) [H00005358-M01] - Analysis of monoclonal antibody to PLS3 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: T Plastin Antibody (5B9) [H00005358-M01] - PLS3 monoclonal antibody (M01), clone 5B9. Analysis of PLS3 expression in MCF-7.

Western Blot: T Plastin Antibody (5B9) [H00005358-M01] - PLS3 monoclonal antibody (M01), clone 5B9. Analysis of PLS3 expression in MDA-MB-231.

ELISA: T Plastin Antibody (5B9) [H00005358-M01] - Detection limit for recombinant GST tagged PLS3 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

T Plastin Antibody (5B9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PLS3 (AAH39049, 1 a.a. ~ 630 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDEMATTQISKDELDELKEAFAKVDLNSNGFICDYELHELFKEANMPLPGYKVREIIQKLMLDGDRNKDGKISFDEFVYIFQEVKSSDIAKTFRKAINRKEGICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRWANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDLKRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVANLFNKYPALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLYADLQDALVILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKHPAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGRGMKRV
• Specificity: This product is specific for Human PLS3 monoclonal antibody (M01), clone 5B9 [Gene ID: 5358].
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLS3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for T Plastin Antibody (5B9) - Azide and BSA Free

• plastin 3 (T isoform)
• plastin 3
• plastin-3
• T plastin
• T-plastinT fimbrin

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T Plastin Antibody (H00005358-M01)(1)

Publication using H00005358-M01

• Garbett D, Bisaria A, Yang C et al. T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration Nat Commun 2020-09-23 [PMID: 32968060] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: PLS3
• Uniprot:
  • AAH39049(Human)