SYTL4 Antibody - BSA Free

Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human endometrium shows weak cytoplasmic positivity in glandular cells.

Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & microtubule organizing center.

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SYTL4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SYTL4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RLKNELLEIKRKGAKRGSQHYSDRTCARCQESLGRLSPKTNTCRGCNHLVCRDCRIQESNGTWRCKVCAKEIELKKATGDWFYDQKVNRFAYRTGSEIIRMSLRHKPAVSKRETVGQSLLHQTQMGDIWPGRKIIQERQKEPSVLFEVP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYTL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SYTL4 Protein (NBP1-86174PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SYTL4 Antibody - BSA Free

• DKFZp451P0116
• exophilin-2
• FLJ40960
• Granuphilin
• granuphilin-a
• SLP4
• synaptotagmin-like 4
• synaptotagmin-like protein 4

Background

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary.Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYTL4