MYRIP Antibody

Images

 
Western Blot: MYRIP Antibody [NB100-1278] - Staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.3ug/ml)
Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MYRIP Antibody Summary

Immunogen
Peptide with sequence C-KDLMEPALESAVMY corresponding to C-Terminus according to NP_056275.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYRIP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 90 kDa band observed in human brain (amygdala) lysates (calculated MW of 95.7 kDa band according to NP_056275.2).
IHC: Paraffin embedded Human Breast, Colon and Brain (Cortex).
Control
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Human Testis Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1278.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MYRIP Antibody

  • DKFZp586F1018
  • exophilin-8
  • FLJ44025
  • MGC130034
  • myosin VIIA and Rab interacting protein
  • Myosin-VIIa- and Rab-interacting protein
  • MyRIP
  • rab effector MyRIP
  • SLAC2-C
  • SLAC2CMGC130035
  • Slp homolog lacking C2 domains c
  • Slp homologue lacking C2 domains
  • synaptotagmin-like protein homologue lacking C2 domains-c

Background

A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MYRIP Antibody (NB100-1278)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYRIP
Entrez
Uniprot