SHD Antibody

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Product Details

Summary
Reactivity DrSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SHD Antibody Summary

Immunogen
Synthetic peptide taken form the Spook protein form within the N-terminal region (amino acid 51-60) the peptide was post-synthetically modified and conjugated to carrier protein to achieve desired antigenicity. The Shade peptides sequences are conserved various other specieis of fruit flys.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHD
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Western Blot 1:500
Application Notes
NBP1-78136 is ideal for WB and IHC applications, other applications have not been tested. This antibody does not cross react to other fly proteins. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay.
Publications
Read Publication using NBP1-78136.

Reactivity Notes

The species cross reactivity for this antibody is not examined fully.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SHD Antibody

  • SH2 domain-containing adapter protein D
  • Src homology 2 domain containing transforming protein D
  • src homology 2 domain-containing transforming protein D

Background

Transposable elements are found in the genomes of nearly all eukaryotes. The recent completion of the Release 3 euchromatic genomic sequence of Drosophila melanogaster by the Berkeley Drosophila Genome Project has provided precise sequence for the repetitive elements in the Drosophila euchromatin. The genomic sequence to describe the euchromatic transposable elements in the sequenced strain of this species identified several new genes. Out of 85 unknown and 8 novel family of transposable elements varying in copy number form 1-146 have been identified. A total of 1500 full and partial transposable elements were identified that make up about 4% of the fruit fly genome. Most of these transposable elements reside outside genes (1). Spook or cytochrome P450a1 enzymes are involved in the detoxification of alkaloids and in xenobiotics resistance in insects. Cytochrome P450s form a large and diverse family of heme-containing proteins capable of carrying out many different enzymatic reactions. Some P450 proteins carry out reaction that are essential for process such as hormone synthesis, while others are P450 enzymes are involved in detoxification of environmental compounds. Shade expression patterns in embryos and s stage of third instar larvae indicate the protein is in abundance in fat bodies, malpighian tubules and in distinct regions of the midgut, consistent with its role in detoxification (2). The biological functions also involve embryonic development via syncytical blastderm, ecdysone biosynthesis process, heat involution, cuticle development. Shade gene sequence location is at 3L:5583181..5585677 is a 540 aa (65kDa) protein. There are at least 14 alleles are reported for this gene. The D. melanogaster Shade-selective antibodies were generated against unique antigenic sequence taken form the N-terminal region with in the amino acids 1-60 and from 175-225. The sequence was conserved in several species of fruit flies, but was unique to shade/CYP341a1 protein and was not present in other proteins. The Shade antibodies were affinity purified over immobilized antigen based chromatography, and the affinity purified immunoglobulins are stabilized in antibody stabilization buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SHD Antibody (NBP1-78136)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SHD Antibody (NBP1-78136)

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Bioinformatics

Gene Symbol SHD
Uniprot