SYPL2 Antibody - BSA Free

Western Blot: SYPL2 Antibody [NBP1-77362] - Human spleen tissue lysate with SYPL2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: SYPL2 Antibody - BSA Free [NBP1-77362] - Immunohistochemistry of SYPL2 in human spleen tissue with SYPL2 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: SYPL2 Antibody - BSA Free [NBP1-77362] - Immunofluorescence of SYPL2 in Human Spleen cells with SYPL2 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SYPL2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SYPL2 Antibody - BSA Free (NBP1-77362) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL. The immunogen is located within the last 50 amino acids of SYPL2. Amino Acid Squence: DQGQGPSQESAAEQG
• Specificity: At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.
• Predicted Species: Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYPL2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  SYPL2 Peptide (NBP1-77362PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SYPL2 Antibody - BSA Free

• MG29
• mitsugumin-29
• synaptophysin-like 2
• synaptophysin-like protein 2

Background

SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores. At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYPL2
• Uniprot:
  • NP_001035799(Human)