CACNA1S Antibody (1A) - BSA Free

Images

 
Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) [NB300-542] - Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue.
Flow Cytometry: CACNA1S Antibody (1A) [NB300-542] - Flow cytometry analysis of Dihydropyridine Receptor alpha-1 in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Gp, RbSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P, IP
Clone
1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

CACNA1S Antibody (1A) - BSA Free Summary

Immunogen
Purified rabbit muscle T-tubule dihydropyridine receptor.
Localization
Plasma membrane
Specificity
Detects 1,4-dihydropyridine (DHP) receptor alpha-1 subunit in skeletal muscle. The DHP Receptor alpha-1 protein is also known as CACNA1S or Cav1.1 alpha-1 subunit.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CACNA1S
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500
Application Notes
WB: Detects an approx. 200 kDa protein representing the DHP receptor in rat skeletal muscle extracts. IHC: Staining of DHP receptor in rabbit skeletal muscle with this antibody results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.
Publications
Read Publications using
NB300-542 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CACNA1S Antibody (1A) - BSA Free

  • CACH1
  • CACN1
  • CACNA1S
  • CACNL1A3
  • CACNL1A3MHS5
  • calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle
  • Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle
  • calcium channel, voltage-dependent, L type, alpha 1S subunit
  • Cav1.1
  • CCHL1A3
  • dihydropyridine receptor
  • dihydropyridine-sensitive L-type calcium channel alpha-1 subunit
  • HOKPP
  • HOKPP1
  • hypokalemic periodic paralysis)
  • hypoPP
  • MHS5
  • TTPP1
  • voltage-dependent L-type calcium channel subunit alpha-1S
  • Voltage-gated calcium channel subunit alpha Cav1.1

Background

Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CACNA1S Antibody (NB300-542)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow, IHC, WB.


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(1)
IHC
(1)
WB
(2)
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(2)
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CACNA1S Antibody (NB300-542). (Showing 1 - 1 of 1 FAQs).

  1. Is there any cross-reactivity data available for this antibody (NB300-542) with CACNA2D2?
    • We have no direct cross-reactivity testing data but a sequence alignment of these two proteins shows less than 10% sequence similarity, so it is not predicted to cross-react.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CACNA1S
Entrez