Syntaxin-BP1 Antibody

Images

 
Western Blot: Syntaxin-BP1 Antibody [NBP1-83139] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: Syntaxin-BP1 Antibody [NBP1-83139] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human cerebellum shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Analysis in human cerebral cortex and liver tissues using NBP1-83139 antibody. Corresponding STXBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human cerebellum shows strong positivity in molecular layer.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Syntaxin-BP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPT
Specificity
Specificity of human Syntaxin-BP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STXBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Syntaxin-BP1 Protein (NBP1-83139PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Syntaxin-BP1 Antibody

  • EIEE4
  • FLJ37475
  • hUNC18
  • MUNC18-1
  • NSEC1
  • N-Sec1
  • p67
  • Protein unc-18 homolog 1
  • Protein unc-18 homolog A
  • rbSec1
  • STXBP1
  • syntaxin binding protein 1
  • syntaxin-binding protein 1
  • SyntaxinBP1
  • Syntaxin-BP1
  • UNC18
  • unc18-1
  • UNC18A
  • unc-18A
  • UNC18neuronal SEC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol STXBP1