Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

Western Blot: Syndecan-4 Antibody (V60P2C2/F2) [NBP2-50315] - Analysis of Syndecan-4 in Hela cell lysate.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: V60P2C2*F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Syndecan-4 Antibody (V60P2C2*F2) - BSA Free Summary

• Description: Novus Biologicals Mouse Syndecan-4 Antibody (V60P2C2*F2) - BSA Free (NBP2-50315) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide Sequence - EDVVGPGQE
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SDC4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): ELISA - Peptide immunogen; WB - Hela whole cell lysate. Product observed is larger than expected- could be due to post translational modifications as Syndecans are heavily glycosylated; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

• Amphiglycan
• MGC22217
• Ryudocan core protein
• Ryudocan
• SDC4
• SYND4ryudocan amphiglycan
• syndecan 4 (amphiglycan, ryudocan)
• syndecan 4
• syndecan proteoglycan 4
• Syndecan4
• Syndecan-4

Background

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDC4