Syndecan-1/CD138 Antibody (MI15)

Product Discontinued

Syndecan-1/CD138 Antibody (MI15) Summary

• Immunogen: Mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with XG1 and U266 myeloma cell lines.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SDC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10-20 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1-5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: For Flow Cytometry: Heat treatment is necessary for paraffin embedded sections. Microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5). Use in ICC/IF was reported in the scientific literature (PMID: 23807541).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Syndecan-1/CD138 Antibody (MI15)

• CD138 antigen
• CD138
• SDC
• SDC1
• SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
• syndecan 1
• syndecan proteoglycan 1
• syndecan
• Syndecan1
• Syndecan-1

Background

The DL-101 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syndecan-1/CD138 Antibody (NBP1-54523)(1)

Publication using NBP1-54523

• Szabo S, Xu Y, Romero R et al. Changes of placental syndecan-1 expression in preeclampsia and HELLP syndrome. Virchows Arch 2013-06-27 [PMID: 23807541] (IF/IHC, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SDC1
• Entrez:
  • 6382(Human)
• Uniprot:
  • P18827(Human)