SYK Antibody (SYK-01) - BSA Free

Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control

Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: SYK-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SYK Antibody (SYK-01) - BSA Free Summary

• Description: Novus Biologicals Mouse SYK Antibody (SYK-01) - BSA Free (NBP3-09107) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fragment (aa 5-360) of human Syk.
• Specificity: The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SYK Antibody (SYK-01) - BSA Free

• DKFZp313N1010
• EC 2.7.10
• EC 2.7.10.2
• FLJ25043
• spleen tyrosine kinaseFLJ37489
• SYK
• tyrosine-protein kinase SYK

Background

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYK