SYK Antibody (SYK-01) - BSA Free

Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)

Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP, KO
• Clone: SYK-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

SYK Antibody (SYK-01) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse SYK Antibody (SYK-01) - BSA Free (NBP1-03250) is a monoclonal antibody validated for use in IHC, WB and IP. Anti-SYK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human SYK.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Western Blot 1:500
• Application Notes: IHC: NBP1-03250 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for SYK Antibody (SYK-01) - BSA Free

• DKFZp313N1010
• EC 2.7.10
• EC 2.7.10.2
• FLJ25043
• spleen tyrosine kinaseFLJ37489
• SYK
• tyrosine-protein kinase SYK

Background

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SYK Antibody (NBP1-03250)(1)

Publication using NBP1-03250

• Alshafie, W;Fotouhi, M;Ayoubi, R;Southern, K;Laflamme, C;NeuroSGC/YCharOS collaborative group, ; Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence F1000Research 2023-10-09 [PMID: 38948505]

Bioinformatics

• Gene Symbol: SYK
• Entrez:
  • 20963(Mouse)
  • 25155(Rat)
• Uniprot:
  • P43405()