SYK Antibody (M373)

Western Blot: SYK Antibody (M373) [NBP3-23427] - Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-SYK (Central region) antibody at 1:250.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M373
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SYK Antibody (M373) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M373 was generated from a sequence corresponding to amino acids in the central region of human Syk. This sequence has high homology to mouse and rat Syk. [UniProt# P43405]
• Specificity: This antibody detects a 72 kDa* protein corresponding to the molecular mass of Syk on SDS-PAGE immunoblots of adult mouse spleen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for SYK Antibody (M373)

• DKFZp313N1010
• EC 2.7.10
• EC 2.7.10.2
• FLJ25043
• spleen tyrosine kinaseFLJ37489
• SYK
• tyrosine-protein kinase SYK

Background

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYK