Surfactant Protein A Antibody

Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - Sca-1+Abcg1+cells are the cancer stem cell-like subset of AT2 cells. Confocal analysis of SPA/ABCG1 and alpha6/beta4 in spheroid of Sca-1+Abcg1+cells isolated from KO mice, (Bar = 10 um). Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41388-020-1251-2) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928] - Staining of paraffin embedded Human Lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes the phosphorylation and acetylation of NF-kB and mediates the elevation of ABCG1 expression in AT2 cells. IF analysis of ABCG1 in the S/TB region of lung tissue from WT or KO mice, (Bar = 100 um). Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41388-020-1251-2) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes NF-kB-mediated ECM1 expression elevation in Lgr5 cells and secreted ECM1-induced AT2 cell enrichment in the S/TB region. IF analysis of AT2 SPA (Surfactant Protein A) cells in the lung S/TB region after KO:Lgr5 cells with ECM1 or p65 knockdown and injected into NOD/SCID mice through tail vein, (Bar = 100 um); Data were collected from three independent experiments with triplicate samples. **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41388-020-1251-2) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - ECM1-alpha 6 beta 4-ABCG1 axis is enriched in the S/TB region of the lungs in patients with pneumonia.IF analysis of GPRC5A, SPA/ABCG1, alpha 6/ beta 4, alpha 6/ECM1 & beta 4/ECM1 in normal or pneumonic lung tissue samples (a) & the calculation of positive or dual-positive cells in the S/TB region (b), (Bar = 100 µm); c Graphical abstract of the study: GPRC5A deficiency promotes the activation of NF-kB & subsequent expression & secretion of ECM1; the secreted ECM1 interacts with alpha 6 beta 4 of AT2 cells & induces the activation of NF-kB, which induces the expression of ABCG1. AT2 cells with ABCG1 expression are one of the originating cell populations of lung cancer. Data were collected from three independent experiments with triplicate samples. **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Surfactant Protein A Antibody Summary

• Description: Novus Biologicals Goat Surfactant Protein A Antibody (NBP2-12928) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Surfactant Protein A Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HLDEELQATLHDFR corresponding to internal region according to NP_005402.3, NP_001087239.2, NP_001158117.1, NP_001158118.1, NP_001092138.1.
• Epitope: CHLDEELQATLHDFR
• Specificity: This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SFTPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human lung lysates (calculated MW of 27.7 kDa band according to NP_001087239.2). Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32157214).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. Use in Mouse reported in scientific literature (PMID:32157214).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Surfactant Protein A Antibody

• COLEC4
• PSAP
• PSPA
• PSP-A
• SFTP1
• SFTPA1
• SFTPA1B
• SPA
• SP-A
• SPA1
• SP-A1
• surfactant protein A1B

Background

Surfactant protein A is a protein with two isoforms, with lengths of 248 and 263 amino acids and weights of approximately 26 and 28 kDa respectively. Surfactant protein A binds to surfactant phospholipids in the presence of calcium ions and helps lower the surface tension in the alveoli of the lungs. Current research is being done on several diseases and disorders linked to this protein including pulmonary fibrosis, allergic bronchopulmonary aspergillosis, pulmonary alveolar proteinosis, non-small cell lung carcinoma, sclerosing hemangioma, otitis media, chronic obstructive pulmonary disease, bronchitis obliterans, bronchopulmonary dysplasia, rhinitis, aspergillosis, pulmonary edema, insulin resistance, and pneumoconiosis. Surfactant protein A has also been shown to have interactions with DMBT1, MYO18A, C1QA, SFTPD, and TLR2 in pathways such as the immune response, bacterial infections in CF airways, cell-cell communication, phagosome, and pertussis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Surfactant Protein A Antibody (NBP2-12928)(3)

Publications using NBP2-12928

• Yin H, Jiang Z, Feng X et Al. Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells Oncogene 2020-03-10 [PMID: 32157214] (IF, Mouse)
• Kosmider B, Lin C, Karim L et al. Mitochondrial dysfunction in human primary alveolar type II cells in emphysema EBioMedicine 2019-08-01 [PMID: 31383554] (IHC-P, Human)
• Benlhabib H, Mendelson CR. Epigenetic regulation of surfactant protein A gene (SP-A) expression in fetal lung reveals a critical role for Suv39h methyltransferases during development and hypoxia. Mol Cell Biol 2011-05-01 [PMID: 21402781]

Bioinformatics

• Gene Symbol: SFTPA1
• Entrez:
  • 653509(Human)