Surfactant Protein A Antibody

Images

 
Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - Sca-1+Abcg1+cells are the cancer stem cell-like subset of AT2 cells. Confocal analysis of SPA/ABCG1 and alpha6/beta4 in spheroid of ...read more
Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928] - Staining of paraffin embedded Human Lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes the phosphorylation and acetylation of NF-kB and mediates the elevation of ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes NF-kB-mediated ECM1 expression elevation in Lgr5 cells and secreted ...read more
Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - ECM1-alpha 6 beta 4-ABCG1 axis is enriched in the S/TB region of the lungs in patients with pneumonia.IF analysis of GPRC5A, ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Surfactant Protein A Antibody Summary

Description
Novus Biologicals Goat Surfactant Protein A Antibody (NBP2-12928) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Surfactant Protein A Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Peptide with sequence C-HLDEELQATLHDFR corresponding to internal region according to NP_005402.3, NP_001087239.2, NP_001158117.1, NP_001158118.1, NP_001092138.1.
Epitope
CHLDEELQATLHDFR
Specificity
This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFTPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human lung lysates (calculated MW of 27.7 kDa band according to NP_001087239.2). Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32157214).
Publications
Read Publications using
NBP2-12928 in the following applications:

  • IF
    1 publication
  • 1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. Use in Mouse reported in scientific literature (PMID:32157214).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Surfactant Protein A Antibody

  • COLEC4
  • PSAP
  • PSPA
  • PSP-A
  • SFTP1
  • SFTPA1
  • SFTPA1B
  • SPA
  • SP-A
  • SPA1
  • SP-A1
  • surfactant protein A1B

Background

Surfactant protein A is a protein with two isoforms, with lengths of 248 and 263 amino acids and weights of approximately 26 and 28 kDa respectively. Surfactant protein A binds to surfactant phospholipids in the presence of calcium ions and helps lower the surface tension in the alveoli of the lungs. Current research is being done on several diseases and disorders linked to this protein including pulmonary fibrosis, allergic bronchopulmonary aspergillosis, pulmonary alveolar proteinosis, non-small cell lung carcinoma, sclerosing hemangioma, otitis media, chronic obstructive pulmonary disease, bronchitis obliterans, bronchopulmonary dysplasia, rhinitis, aspergillosis, pulmonary edema, insulin resistance, and pneumoconiosis. Surfactant protein A has also been shown to have interactions with DMBT1, MYO18A, C1QA, SFTPD, and TLR2 in pathways such as the immune response, bacterial infections in CF airways, cell-cell communication, phagosome, and pertussis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Surfactant Protein A Antibody (NBP2-12928)(3)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SFTPA1
Entrez