Surfactant Protein A Antibody

Images

 
Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - Sca-1+Abcg1+cells are the cancer stem cell-like subset of AT2 cells. Confocal analysis of SPA/ABCG1 and alpha6/beta4 in spheroid of ...read more
Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928] - Staining of paraffin embedded Human Lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes the phosphorylation and acetylation of NF-kB and mediates the elevation of ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - GPRC5A deficiency promotes NF-kB-mediated ECM1 expression elevation in Lgr5 cells and secreted ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Surfactant Protein A Antibody Summary

Immunogen
Peptide with sequence C-HLDEELQATLHDFR corresponding to internal region according to NP_005402.3, NP_001087239.2, NP_001158117.1, NP_001158118.1, NP_001092138.1.
Epitope
CHLDEELQATLHDFR
Specificity
This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFTPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human lung lysates (calculated MW of 27.7 kDa band according to NP_001087239.2). Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32157214).
Publications
Read Publications using
NBP2-12928 in the following applications:

  • IF
    1 publication
  • 1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. Use in Mouse reported in scientific literature (PMID:32157214).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Surfactant Protein A Antibody

  • COLEC4
  • PSAP
  • PSPA
  • PSP-A
  • SFTP1
  • SFTPA1
  • SFTPA1B
  • SPA
  • SP-A
  • SPA1
  • SP-A1
  • surfactant protein A1B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Surfactant Protein A Antibody (NBP2-12928)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SFTPA1
Entrez