Surfactant Protein A Antibody (6F10) - BSA Free

Immunohistochemistry-Paraffin: Surfactant Protein A Antibody (6F10) [NBP2-41344] - Analysis using the Biotin conjugate of NBP2-41344. Staining of sections of human lung

Immunohistochemistry-Paraffin: Surfactant Protein A Antibody (6F10) [NBP2-41344] - Analysis using the Biotin conjugate of NBP2-41344. Staining of sections of human lung

Product Discontinued

Surfactant Protein A Antibody (6F10) - BSA Free Summary

• Immunogen: Native SP-A purified from bronchioaveolar lavage from proteinosis patients
• Specificity: Specific for human pulmonary Surfactant protein A (SP-A).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SFTPA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Microarray
  • Protein Array
  • Western Blot 1:1000
• Application Notes: Use in Microarray reported in scientific literature (PMID 24009888). Use in Protein Array reported in scientific literature (PMID 25735706). ELISA: NBP2-41344 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well. WB: In Western blotting after SDS-PAGE NBP2-41344 reacts with reduced forms of SP-A and weaker with non-reduced forms. A dilution guideline of 1/1000 has proved successful. (2) IHC: In immunohistochemical staining from paraffin embedded tissue samples, NBP2-41344 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from the pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. (1, 2)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Surfactant Protein A Antibody (6F10) - BSA Free

• COLEC4
• PSAP
• PSPA
• PSP-A
• SFTP1
• SFTPA1
• SFTPA1B
• SPA
• SP-A
• SPA1
• SP-A1
• surfactant protein A1B

Background

Surfactant protein A (SP-A) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-D and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45 ng/ml in healthy individuals (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Surfactant Protein A Antibody (NBP2-41344)(6)

Publications using NBP2-41344

• Ghosh A, Ahmad S, Coakley RD et al. Lipid Laden Macrophages are not Unique to EVALI Patients American journal of respiratory and critical care medicine 2020-12-17 [PMID: 33332247]
• Jung AL, Moller Jorgensen M, Baek R et al. Surface Proteome of Plasma Extracellular Vesicles as Biomarkers for Pneumonia and Acute Exacerbation of Chronic Obstructive Pulmonary Disease J. Infect. Dis. 2019-10-16 [PMID: 31617573] (Human)
• Baek R, Varming K, Jorgensen MM. Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma?. Transfus. Apher. Sci. 2016-08-01 [PMID: 27470710]
• Sandfeld-Paulsen B, Jakobsen KR, Baek R et al. Exosomal Proteins as Diagnostic Biomarkers in Lung Cancer J Thorac Oncol 2016-06-22 [PMID: 27343445]
• Jakobsen KR, Paulsen BS, Baek R et al. Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma J Extracell Vesicles 2015-03-04 [PMID: 25735706] (PA, Human)
  
  Details:
  Surfactant Protein A antibody (clone 6F10) used in Extracellular Vesicle Array /EV Array application for phenotyping exosomes directly from the plasma samples of non-small cell lung carcinoma/ NSCLC patients with advanced stage (IIIa-IV) disease and matched control subjects initially suspected of having cancer, but diagnosed to be cancer free. The array implicated 37 antibodies against lung cancer-related proteins.
• Jorgensen M, Baek R, Pedersen S et al. Extracellular Vesicle (EV) Array: microarray capturing of exosomes and other extracellular vesicles for multiplexed phenotyping J Extracell Vesicles 2013-06-18 [PMID: 24009888] (MiAr, Human)
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: SFTPA1