SUMO3 Overexpression Lysate

Images

 
Western Blot: Sumo 3 Overexpression Lysate (Adult Normal) [NBL1-16613] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sumo 3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SUMO3 Overexpression Lysate Summary

Description

SUMO3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200241

Accession#: NM_006936

Protein Tag: C-MYC/DDK
Gene
SUMO3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
11.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SUMO3 Overexpression Lysate

  • SMT3 (suppressor of mif two 3, yeast) homolog 1
  • SMT3 homolog 1
  • SMT3 suppressor of mif two 3 homolog 1
  • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 3 (yeast)
  • SMT3A
  • SMT3B
  • SMT3H1
  • SMT3H1small ubiquitin-related modifier 3
  • SUMO-2
  • SUMO3
  • SUMO-3
  • ubiquitin-like protein SMT3A
  • Ubiquitin-like protein SMT3B

Background

SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SUMO3