SUMO3 Antibody (JJ087-04)

Western Blot: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Western blot analysis of SUMO3 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra

Immunocytochemistry/Immunofluorescence: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Staining Sumo 2+3 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-SUMO3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Staining Sumo 2+3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Staining Sumo 2+3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded human kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse lung tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse skin tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-SUMO3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SUMO3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded rat skin tissue with Rabbit anti-SUMO3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

SUMO3 Antibody (JJ087-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SUMO3 aa 11-60 / 103. (SwissProt: P55854 Human; SwissProt: P61956 Human; SwissProt: P61957 Mouse; SwissProt: Q9Z172 Mouse; SwissProt: P61959 Rat; SwissProt: Q5XIF4 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SUMO3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SUMO3 Antibody (JJ087-04)

• SMT3 (suppressor of mif two 3, yeast) homolog 1
• SMT3 homolog 1
• SMT3 suppressor of mif two 3 homolog 1
• SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 3 (yeast)
• SMT3A
• Smt3B
• SMT3H1
• SMT3H1small ubiquitin-related modifier 3
• SUMO-2
• SUMO3
• SUMO-3
• ubiquitin-like protein SMT3A
• Ubiquitin-like protein SMT3B

Background

SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUMO3