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SUMO3 Antibody (JJ087-04)

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Western Blot: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Western blot analysis of SUMO3 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra
Western Blot: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Western blot analysis of SUMO3 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. ...read more
Immunocytochemistry/ Immunofluorescence: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Staining Sumo 2+3 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse skin tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Staining Sumo 2+3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunocytochemistry/ Immunofluorescence: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Staining Sumo 2+3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded human kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SUMO3 Antibody (JJ087-04) [NBP2-67842] - Analysis of paraffin-embedded mouse lung tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
JJ087-04
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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SUMO3 Antibody (JJ087-04) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human Sumo 3 aa 20-60. (SwissProt: P55854 Human;SwissProt: P61956 Human;SwissProt: P61957 Mouse;SwissProt: Q9Z172 Mouse;SwissProt: P61959 Rat;SwissProt: Q5XIF4 Rat)
Localization
Cytoplasm, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SUMO3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SUMO3 Antibody (JJ087-04)

  • SMT3 (suppressor of mif two 3, yeast) homolog 1
  • SMT3 homolog 1
  • SMT3 suppressor of mif two 3 homolog 1
  • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 3 (yeast)
  • SMT3A
  • SMT3B
  • SMT3H1
  • SMT3H1small ubiquitin-related modifier 3
  • SUMO-2
  • SUMO3
  • SUMO-3
  • ubiquitin-like protein SMT3A
  • Ubiquitin-like protein SMT3B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Human Tonsil stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
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Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SJ20-03) [NBP2-67419] - Staining SUMO-1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SJ20-03) [NBP2-67419] - Staining SUMO-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Jurkat cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RanGAP1 antibody, and then analyzed by flow cytometry.
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Western Blot: SENP2 Antibody (3D3) [NBP2-03852] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SENP2.
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SUMO3 Antibody
(JJ087-04)
NBP2-67842
SUMO3 Antibody (JJ087-04)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol SUMO3