SUMO3 Antibody (907614)

Western blot shows lysates of SUMO3. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO3 Monoclonal Antibody (Catalog # A-720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO3 at approximately 16-18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

SUMO3 Antibody (907614) Summary

• Immunogen: Peptide sequence from SUMO3
  Accession # P55854
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SUMO3

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.
• Buffer: Supplied as a solution in PBS containing Glycerol and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SUMO3 Antibody (907614)

• SMT3 (suppressor of mif two 3, yeast) homolog 1
• SMT3 homolog 1
• SMT3 suppressor of mif two 3 homolog 1
• SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 3 (yeast)
• SMT3A
• SMT3B
• SMT3H1
• SMT3H1small ubiquitin-related modifier 3
• SUMO-2
• SUMO3
• SUMO-3
• ubiquitin-like protein SMT3A
• Ubiquitin-like protein SMT3B

Background

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO3, also known as SMT3A, is synthesized as a 103 amino acid (aa), 11.5 kDa propeptide that contains an 11 aa C­terminal prosegment. Following prosegment cleavage, the C­terminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO3 shares 83% sequence identity with SUMO3 from mouse. SUMO3 also has very high sequence homology to SUMO2 and SUMO4, 87% and 75%, respectively. SUMO3 shares only 47% sequence identity to SUMO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUMO3
• Uniprot:
  • P55854()