Sulfatase Modifying Factor 1/SUMF1 Antibody

Western Blot: Sulfatase Modifying Factor 1/SUMF1 Antibody [NBP1-45212] - Analysis of SUMF1 in Mouse Pancreas lysate (35ug protein in RIPA buffer) using NBP1-45212 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Sulfatase Modifying Factor 1/SUMF1 Antibody Summary

• Description: Novus Biologicals Goat Sulfatase Modifying Factor 1/SUMF1 Antibody (NBP1-45212) is a polyclonal antibody validated for use in WB and ELISA. Anti-Sulfatase Modifying Factor 1/SUMF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ETLNPKGPPSGKDR corresponding to internal region according to NP_877437.2.
• Epitope: internal region
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SUMF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 37-40 kDa band observed in mouse pancreas, mouse eye and human kidney lysates (calculated MW of 40.6 kDa band according to human NP_877437.2 and 40.6 kDa band according to mouse NP_666049.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sulfatase Modifying Factor 1/SUMF1 Antibody

• AAPA3037
• EC 1.8.99.-
• FGE1
• FGEC-alpha-formylglycine-generating enzyme 1
• FGly-generating enzyme
• MGC131853
• MGC150436
• sulfatase modifying factor 1
• sulfatase-modifying factor 1
• SUMF1
• UNQ3037

Background

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD; MIM 272200), a lysosomal storage disorder (Roeser et al., 2006 [PubMed 16368756]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sulfatase Modifying Factor 1/SUMF1 Antibody (NBP1-45212)(1)

Publication using NBP1-45212

• Oshikawa M, Usami R, Kato S. Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19. Mol Vis 2009-01-01 [PMID: 19262745]

Bioinformatics

• Gene Symbol: SUMF1
• Entrez:
  • 285362(Human)
  • 58911(Mouse)
  • 362409(Rat)
• Uniprot:
  • NP_877437.2(Human)