| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57621-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22583-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35540-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35357-20ul | |||
![Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/SUG1-Antibody-25D5-Western-Blot-NB100-345-img0002.jpg "Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000")
![<b>Genetic Strategies Validation. </b>Western Blot: SUG1 Antibody (25D5) [NB100-345] - Representative immunoblot for SUG1 antibody knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC3695522/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUG1-Antibody-25D5-Knockdown-Validated-NB100-345-img0003.jpg)
![Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SUG1 antibody [25D5] (NB100-345) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-345_mouse-monoclonal-sug1-antibody-25d5-western-blot-141120251832290.jpg)
![Western Blot: SUG1 Antibody [NBP1-87372] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NBP1-87372-img0009.jpg "Western Blot: SUG1 Antibody [NBP1-87372] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: SUG1 Antibody [NBP1-87372] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87372-img0010.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human testis.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-87372-img0014.jpg)
![Western Blot: SUG1 Antibody [NBP1-87372] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NBP1-87372-img0008.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human adrenal gland shows nuclear and cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-87372-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human cerebral cortex, lymph node, skin and testis using Anti-PSMC5 antibody NBP1-87372 (A) shows similar protein distribution across tissues to independent antibody NBP2-57621 (B).](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-87372-img0011.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human lymph node.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-87372-img0012.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human skin.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-87372-img0013.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-87372-img0015.jpg)
![Western Blot: SUG1 Antibody [NBP1-33309] - Sample (30 ug of whole cell lysate) A: A431 B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NBP1-33309-img0008.jpg "Western Blot: SUG1 Antibody [NBP1-33309] - Sample (30 ug of whole cell lysate) A: A431 B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SUG1 Antibody [NBP1-33309] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33309-img0004.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-33309] - Paraffin-embedded human hepatocellular carcinoma. PSMC5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP1-33309-img0009.jpg)
![Western Blot: SUG1 Antibody [NBP1-33309] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract, C. 30 ug C8D30 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NBP1-33309-img0006.jpg)
![Western Blot: SUG1 Antibody [NBP1-33309] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NBP1-33309-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SUG1 Antibody [NBP2-57621] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57621-img0001.jpg "Immunocytochemistry/Immunofluorescence: SUG1 Antibody [NBP2-57621] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-57621] - Staining of human testis.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-57621-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-57621] - Staining of human cerebral cortex, lymph node, skin and testis using Anti-PSMC5 antibody NBP2-57621 (A) shows similar protein distribution across tissues to independent antibody NBP1-87372 (B).](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-57621-img0004.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-57621] - Staining of human lymph node.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-57621-img0005.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-57621] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-57621-img0006.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-57621] - Staining of human skin.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-57621-img0008.jpg)
![Western Blot: SUG1 Antibody [NBP2-20529] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NBP2-20529-img0001.jpg "Western Blot: SUG1 Antibody [NBP2-20529] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: SUG1 Antibody [NBP2-20529] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20529-img0003.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-20529] - SUG1 antibody detects SUG1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SUG1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-20529-img0004.jpg)
![Immunohistochemistry-Paraffin: SUG1 Antibody [NBP2-20529] - Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-Paraffin-NBP2-20529-img0002.jpg)
![Western Blot: SUG1 Antibody [NB100-2572] - Detection of Human and Mouse SUG1 on HeLa whole cell lysate (5, 15, and 50 microgram), 293T (T; 50 microgram), and NIH3T3 (M; 50 microgram) using NB100-2572. SUG1 was also immunoprecipitated by rabbit SUG1 antibodies NB100-2573 and another SUG1 Ab.](http://images.novusbio.com/fullsize/SUG1-Antibody-Western-Blot-NB100-2572-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SUG1 Antibody [NB100-2572] - Detection of Human and Mouse SUG1 on HeLa whole cell lysate (5, 15, and 50 microgram), 293T (T; 50 microgram), and NIH3T3 (M; 50 microgram) using NB100-2572. SUG1 was also immunoprecipitated by rabbit SUG1 antibodies NB100-2573 and another SUG1 Ab.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: SUG1 Antibody [NB100-2572] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti- TRIP1(SUG1) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SUG1-Antibody-Immunohistochemistry-NB100-2572-img0008.jpg)
![Western Blot: SUG1 Antibody (SR1803) [NBP3-22583] - Western blot analysis using NBP3-22583 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22583_rabbit-sug1-mab-sr1803-57202315545146.jpg "Western Blot: SUG1 Antibody (SR1803) [NBP3-22583] - Western blot analysis using NBP3-22583 in A431 cell lysate.")
![Western Blot: SUG1 Antibody [NBP3-35540] - Western blot analysis of various lysates using SUG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35540_rabbit-sug1-pab-912202415133477.jpg "Western Blot: SUG1 Antibody [NBP3-35540] - Western blot analysis of various lysates using SUG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/ Immunofluorescence: SUG1 Antibody [NBP3-35540] - Immunofluorescence analysis of MCF-7 cells using SUG1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35540_rabbit-sug1-pab-91220241517525.jpg)
![Western Blot: SUG1 Antibody (PSH0-22) [NBP3-32865] - Western blot analysis of SUG1 on different lysates with Rabbit anti-SUG1 antibody (NBP3-32865) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF-7 cell lysate (20 ug/Lane) Lane 3: Hela cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse testis tissue lysate (40 ug/Lane) Lane 9: Mouse heart tissue lysate (40 ug/Lane) Lane 10: Rat testis tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32865) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32865_rabbit-sug1-mab-psh0-22-288202412442939.jpg "Western Blot: SUG1 Antibody (PSH0-22) [NBP3-32865] - Western blot analysis of SUG1 on different lysates with Rabbit anti-SUG1 antibody (NBP3-32865) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF-7 cell lysate (20 ug/Lane) Lane 3: Hela cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse testis tissue lysate (40 ug/Lane) Lane 9: Mouse heart tissue lysate (40 ug/Lane) Lane 10: Rat testis tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32865) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SUG1 Antibody (PSH0-22) [NBP3-32865] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-SUG1 antibody (NBP3-32865) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32865) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32865_rabbit-sug1-mab-psh0-22-298202414161839.jpg)
![Western Blot: SUG1 Antibody [NBP3-35357] - Western blot analysis of various lysates using SUG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35357_rabbit-sug1-pab-1012202411404075.jpg "Western Blot: SUG1 Antibody [NBP3-35357] - Western blot analysis of various lysates using SUG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: SUG1 Overexpression Lysate (Adult Normal) [NBL1-14892] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SUG1.](http://images.novusbio.com/fullsize/SUG1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14892-img0002.jpg "Western Blot: SUG1 Overexpression Lysate (Adult Normal) [NBL1-14892] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SUG1.")