STYK1 Antibody (484713)

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, DLD clone 2C2 human colon adenocarcinoma cell line, and T47D human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human STYK1 Monoclonal Antibody (Catalog # MAB6668) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STYK1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

STYK1 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human STYK1 Monoclonal Antibody (Catalog # MAB6668) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human STYK1 Monoclonal Antibody (Catalog # MAB6668, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 484713
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

STYK1 Antibody (484713) Summary

• Immunogen: E. coli-derived recombinant human STYK1
  Asp72-Val320
  Accession # Q6J9G0
• Specificity: Detects human STYK1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: STYK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STYK1 Antibody (484713)

• DKFZp761P1010
• EC 2.7.10.2
• NOK
• NOKProtein PK-unique
• serine/threonine/tyrosine kinase 1Novel oncogene with kinase domain
• STYK1
• SuRTK106
• tyrosine-protein kinase STYK1

Background

STYK1 (Ser/Thr/Tyr kinase 1; also NOK, or “novel oncogene with kinase-domain”) is a 50 kDa, distant member of the FGFR/PDGFR family of tyrosine kinases.  It appears to be intracellular, even though it contains a putative transmembrane segment.  Both MAPK and PI3K pathways are activated by STYK1 activity, and STYK1 is associated with oncogenesis.  Human STYK1 is 422 amino acids (aa) in length.  It contains 25 aa N-terminus, a 21 aa putative transmembrane segment, and a 396 aa C-terminus that contains a Tyr-kinase domain (aa 118-372).  Over aa 72-320, human STYK1 is 77% and 83% identical to mouse and canine STYK1, respectively.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STYK1
• Uniprot:
  • Q6J9G0()