STIM1 Antibody (CDN3H4)

Images

 
Western Blot: STIM1 Antibody (CDN3H4) [NB110-55300] - (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining ...read more
Immunocytochemistry/ Immunofluorescence: STIM1 Antibody (CDN3H4) [NB110-55300] - Staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
CDN3H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

STIM1 Antibody (CDN3H4) Summary

Immunogen
Synthetized peptide from C-terminal cytoplasmic part of STIM1. (NM_003156.3).
Specificity
The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STIM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:50
Application Notes
Western Blotting - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions. Immunocytochemistry - Staining technique: methanol-aceton fixation
Reviewed Applications
Read 1 Review rated 4
using
NB110-55300 in the following applications:

Publications
Read Publications using NB110-55300.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for STIM1 Antibody (CDN3H4)

  • GOK
  • GOKD11S4896E
  • STIM1
  • stromal interaction molecule 1

Background

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STIM1 Antibody (NB110-55300)(2)

Review for STIM1 Antibody (NB110-55300) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NB110-55300:
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Images Ratings Applications Species Date Details
Western Blot STIM1 NB110-55300
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reviewed by:
Yuanyuan Tie
WB Rat 11/23/2017
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Summary

ApplicationWestern Blot
Sample TestedH9c2 whole cell lysate
SpeciesRat
Lot526888

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STIM1 Antibody (NB110-55300) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-55300AF405
Alexa Fluor 488 Labeled NB110-55300AF488
Alexa Fluor 647 Labeled NB110-55300AF647
Alexa Fluor 700 Labeled NB110-55300AF700
DyLight 350 Labeled NB110-55300UV
DyLight 405 Labeled NB110-55300V
DyLight 488 Labeled NB110-55300G
DyLight 680 Labeled NB110-55300FR
DyLight 755 Labeled NB110-55300IR

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Recent Reviews

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5
0
4
1
3
0
2
0
1
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Yuanyuan Tie
11/23/2017
Application: WB
Species: Rat

Bioinformatics

Gene Symbol STIM1
Entrez
Uniprot