STI1 Antibody (1C6) - Azide and BSA Free

Western Blot: STI1 Antibody (1C6) [H00010963-M06] - Analysis of STIP1 expression in Raw 264.7.

Immunocytochemistry/Immunofluorescence: STI1 Antibody (1C6) [H00010963-M06] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: STI1 Antibody (1C6) [H00010963-M06] - Analysis of STIP1 expression in PC-12.

Western Blot: STI1 Antibody (1C6) [H00010963-M06] - Analysis of STIP1 expression in NIH/3T3.

Sandwich ELISA: STI1 Antibody (1C6) [H00010963-M06] - Detection limit for recombinant GST tagged STIP1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

STI1 Antibody (1C6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse STI1 Antibody (1C6) - Azide and BSA Free (H00010963-M06) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-STI1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: STIP1 (NP_006810.1, 445 a.a. ~ 543 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
• Specificity: This product is specific for Human STIP1 monoclonal antibody (M06), clone 1C6 [Gene ID: 10963].
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STIP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Mouse monoclonal antibody raised against a partial recombinant STIP1.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STI1 Antibody (1C6) - Azide and BSA Free

• HOP
• HOPSTI1L
• Hsc70/Hsp90-organizing protein
• Hsp70/Hsp90-organizing protein
• IEF-SSP-3521
• NY-REN-11 antigen
• NY-REN-11
• Renal carcinoma antigen NY-REN-11
• STI1
• STI1L
• STI1P60
• STIP1
• stress-induced-phosphoprotein 1
• Transformation-sensitive protein IEF SSP 3521

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 (PubMed 16100115)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STI1 Antibody (H00010963-M06)(1)

Publication using H00010963-M06

• Tsai CL, Tsai CN, Lin CY et al. Secreted Stress-Induced Phosphoprotein 1 Activates the ALK2-SMAD Signaling Pathways and Promotes Cell Proliferation of Ovarian Cancer Cells. Cell Rep. 2012-08-09 [PMID: 22884369]

Bioinformatics

• Gene Symbol: STIP1
• Uniprot:
  • NP_006810(Human)