Recombinant Human Stella/Dppa3 His Protein

SDS-Page: STELLA Protein [NBP2-22997]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Stella/Dppa3 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-159 of Human Stella/Dppa3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDPSQFN PTYIPGSPQM LTEENSRDDS GASQISSETL IKNLSNLTIN ASSESVSPLS EALLRRESVG AAVLREIEDE WLYSRRGVRT LLSVQREKMA RLRYMLLGGV RTHERRPTNK EPKGVKKESR PFKCPCSFCV SNGWDPSENA RIGNQDTKPL QP

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DPPA3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 20.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Stella/Dppa3 His Protein

• developmental pluripotency associated 3
• DPPA3 developmental pluripotency associated 3
• Dppa3
• Stella

Background

DPPA3 is a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this protein may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. Recombinant human DPPA3 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPPA3