Staufen Antibody - BSA Free

Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody diluted at 1:500.Blue: Fluoroshield with DAPI.

Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000

Western Blot: Staufen Antibody [NBP1-33202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Staufen stained by Staufen antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit IgG-IP, Lane E:Post-IP lysate from Stau1-IP; diluted at 1:3000.

Staufen1 protein but not mRNA steady-state levels are increased in neurodegenerative disease cells & tissues. Western blot analysis of SCA2- FBs (a) & LBCs (b) show increased STAU1 levels compared with normal controls. DDX6 levels are unchanged. HD & SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal & five SCA2 FBs, & two normal & three SCA2 LBCs were used. c, d Western blot analyses of ATXN2Q127 (c) & BAC-Q72 (d) mouse cerebellar extracts (24 weeks of age) showing increased Stau1 levels compared with wild-type or BAC-Q22 controls (n = 2–3 animals per group). e Western blot of FB extracts from an ALS patient with the TDP-43G298S mutation show increased STAU1 levels. beta -Actin was used as loading control & representative blots of three independent experiments are shown. f–hSTAU1 RNA levels are unaltered in SCA2 & ALS cells & SCA2 mice. qRT-PCR analyses of STAU1 mRNA in SCA2 FBs & ALS FB with TDP-43G298S mutation (f) or SCA2 LBCs (g). h qRT-PCR analyses of cerebellar RNAs from ATXN2Q127 & BAC-Q72 mice compared to wild-type littermates (24 weeks of age; n = animals per group). Gene expression levels were normalized to Actb Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Staufen Antibody [NBP1-33202] - Silencing of STAU1 mitigates SCA2 phenotypes. aStau1 haploinsufficiency improves abnormal motor behavior of ATXN2Q127 mice as determined by rotarod behavior at 8, 12, 16, & 20 weeks of age. ATXN2Q127;Stau1+/- mice (green) have improved rotarod performance compared with ATXN2Q127 littermates (red) starting at 12 weeks of age. Note that Stau1 haploinsufficiency (orange) by itself does not alter motor function; n = 9–15 mice per group. Values shown are mean ± SE. Significance was determined using generalized estimating equations (GEE). NS, nonsignificant, *P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P < 0.01, Student t-test. d Histograms representing the quantities & sizes of ATXN2-STAU1 co-localized granules in normal vs. SCA2 FBs. e In vivo co-localization of ATXN2 (green) with Stau1 (red) to aggregates in cerebellar PCs of 24-weeks-old ATXN2Q127 mice (white arrows). Scale bar, 30 µM. f Co-localized ATXN2 (red) & STAU1 (green) to aggregates (white arrows) in cerebellar PCs from a human SCA2 brain, that are absent in an unaffected control. Scale bar, 30 µM. g Immunoprecipitation of ATXN2 with STAU1. Non-RNase A or RNase A treated HEK-293 cell extracts expressing Flag-tagged ATXN2-(Q22 or Q108) were subjected to immunoprecipitation with Flag mAb beads & analyzed by western blotting. STAU1 shows RNA-dependent interactions with wild-type & mutant ATXN2. ATXN2 also co-immunoprecipitates with DDX6 & PABPC1, known ATXN2 interactors. Representative blots of three independent experiments are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ch, RM
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Staufen Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Staufen. The exact sequence is proprietary.
• Localization: Rough endoplasmic reticulum
• Predicted Species: Rat (94%), Chicken (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAU1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:500-1:3000
  • Western Blot 1:500-1:3000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (81%), Xenopus laevis (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Staufen Antibody - BSA Free

• double-stranded RNA-binding protein Staufen homolog 1
• FLJ25010
• RNA binding protein (Drosophila)
• RNA-binding protein)
• staufen, RNA binding protein, homolog 1 (Drosophila)

Background

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Staufen Antibody (NBP1-33202)(6)

Publications using NBP1-33202

• Paul S, Dansithong W, Gandelman M et al. Staufen Impairs Autophagy in Neurodegeneration Annals of Neurology 2023-02-01 [PMID: 36151701] (Western Blot, Human)
• Scoles DR, Gandelman M, Paul S et al. A quantitative high throughput screen identifies compounds that lower expression of the SCA2- and ALS-associated gene ATXN2 The Journal of biological chemistry 2022-07-01 [PMID: 35787375]
• Gandelman M, Dansithong W, Kales SC et al. The AKT modulator A-443654 reduces ?-synuclein expression and normalizes ER stress and autophagy Journal of Biological Chemistry 2021-10-01 [PMID: 34520759] (Western Blot, Human)
• Paul S, Dansithong W, Figueroa KP et al. Staufen1 in human neurodegeneration Annals of neurology 2021-03-21 [PMID: 33745139]
• Corbet GA, Burke JM, Bublitz GR, Parker R dsRNA-induced condensation of antiviral proteins promotes PKR activation J Biol Chem 2020-01-04 [PMID: 31896577]
• Paul S, Dansithong W, Figueroa KP et al. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun 2018-09-07 [PMID: 30194296] (WB, Human)

Bioinformatics

• Gene Symbol: STAU1
• Entrez:
  • 6780(Human)
  • 20853(Mouse)
• Uniprot:
  • O95793(Human)