Home » Staufen » Staufen Antibodies » Staufen Antibody

Staufen Antibody

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000
Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: Staufen Antibody [NBP1-33202] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Staufen stained by Staufen antibody ...read more
Western Blot: Staufen Antibody [NBP1-33202] - HepG2 whole cell lysate (30 ug) stained with Staufen antibody, diluted at 1:5000.
Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

Staufen Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Staufen. The exact sequence is proprietary.
Localization
Rough endoplasmic reticulum
Predicted Species
Rat (94%), Chicken (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAU1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:500-1:3000
  • Western Blot 1:500-1:3000
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-33202 in the following applications:

Reactivity Notes

Zebrafish (81%), Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Staufen Antibody

  • double-stranded RNA-binding protein Staufen homolog 1
  • FLJ25010
  • RNA binding protein (Drosophila)
  • RNA-binding protein)
  • staufen, RNA binding protein, homolog 1 (Drosophila)

Background

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

STAU2 Antibody
NBP1-89380
Western Blot: STAU2 Antibody [NBP1-89380] - Analysis in human cell line MOLT-4.
Western Blot: STAU2 Antibody [NBP1-89380] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: STAU2 Antibody [NBP1-89380] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cell bodies and processes.
(1 Publication)
Species: Hu, Rt
Applications: IHC, IHC-P, IP, WB
RENT1/UPF1/hUPF1
Antibody
NB100-368
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RENT1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(10 Publications)
Species: Hu, Mu
Applications: WB
FMRP Antibody
(OTI1C6)
NBP2-01770
Flow Cytometry: FMRP Antibody (1C6) [NBP2-01770] - Analysis of Jurkat cells, using anti-FMRP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: FMRP Antibody (1C6) [NBP2-01770] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMRP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMRP.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FMRP mouse monoclonal antibody.
(1 Review)
(2 Publications)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
RNPC3 Antibody
NBP2-93867
Western Blot: RNPC3 Antibody [NBP2-93867] - Analysis of extracts of various cell lines, using RNPC3 . Exposure time: 60s.
Species: Hu, Mu, Rt
Applications: WB
ARF1 Antibody (1D9)
NB300-505
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of 3T3 cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of Hela cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of MCF-7 cells compared to an isotype control (blue).
(13 Publications)
Species: Bv, Ca, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
IGF2BP1 Antibody
NBP2-38956
Simple Western: IGF2BP1 Antibody [NBP2-38956] - Simple Western lane view shows a specific band for IGF2BP1 in 0.2 mg/ml of HT-29 (left) and HCT 116 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
Simple Western: IGF2BP1 Antibody [NBP2-38956] - Electropherogram image of the corresponding Simple Western lane view. IGF2BP1 antibody was used at 1:10 dilution on HT-29 and HCT 116 lysate(s) respectively.
Western Blot: IGF2BP1 Antibody [NBP2-38956] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line CACO-2
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
CYP4V2 Antibody
(M29-P3B10) - BSA Fre
NBP2-50214
Immunohistochemistry: CYP4V2 Antibody (M29-P3B10) [NBP2-50214] - CYP4V2 neat supernatant.
Western Blot: CYP4V2 Antibody (M29-P3B10) [NBP2-50214] - Shows detection of an approximately 60 kDa band for CYP4V2.
Immunohistochemistry: CYP4V2 Antibody (M29-P3B10) [NBP2-50214] - Immunohistochemistry was performed on formalin-fixed, paraffin-embedded normal and primary colorectal tumour tissue sections using neat culture supernatants of anti-CYP4V2 (M29-P3B10) . Photomicrographs show negative staining of anti-CYP4V2 [M29-P3B10] in normal colon mucosa and strong immunostaining in primary colorectal tumour. Cytoplasmic localisation of CYP4V2 is also observed.
Species: Hu
Applications: ELISA, IHC, WB
CASC3 Antibody
NBP1-30321
Immunohistochemistry: CASC3 Antibody [NBP1-30321] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CASC3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .
Immunoprecipitation: CASC3 Antibody [NBP1-30321] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CASC3 used for IP at 3 mcg/mg lysate. CASC3 was also immunoprecipitated by another rabbit anti-CASC3 antibody, which recognizes a downstream epitope.
Species: Hu
Applications: IHC, IHC-P, IP, WB (-)
VASA Antibody
[Unconjugated]
AF2030
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
(23 Publications)
Species: Hu
Applications: IHC, WB
Serpin E1/PAI-1
[HRP]
DSE100
(4 Reviews)
(37 Publications)
Species: Hu
Applications: ELISA
NUFIP2 Antibody
NB100-79842
Western Blot: NUFIP2 Antibody [NB100-79842] - Detection of Human and Mouse NUFIP2/82-FIP on HeLa whole cell lysate using NB100-79842. NUFIP2/82-FIP was also immunoprecipitated by rabbit anti-NUFIP2/82-FIP antibody NB100-79841.
Species: Hu, Mu
Applications: IP, WB
Signal Peptide
Peptidase Antibody
NBP1-92389
Western Blot: Signal Peptide Peptidase Antibody [NBP1-92389] - Analysis in control (vector only transfected HEK293T lysate) and HM13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Signal Peptide Peptidase Antibody [NBP1-92389] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.
Species: Hu
Applications: IHC, IHC-P, WB
MAP1B [p Thr1265]
Antibody - SuperBUGS
NBP1-42827
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Western blot analysis of p62, Nbr1, MAP1B-HC, and MAP1B-LC1 protein levels following blockage of autophagic protein degradation. Phospho-MAP1B-HC is degraded by autophagy. Upon blockage of autophagic degradation with Bafilomycin A1: (Baf), levels of phospho-MAP1B-HC increase compared with levels of total MAP1B-HC. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ijcb/2012/208014/), licensed under a CC-BY licence.
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
Immunohistochemistry: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.
(8 Publications)
Species: Ch, Fi, Hu, Pm, Mu, Rt, Re, Xp
Applications: ICC/IF, IHC, IHC-Fr, WB
MAP2 Antibody
NB300-213
Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band observed at ~280 kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations.
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Human DA neurons 28 DIV stained with MAP2 antibody (blue). ICC/IF image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:10,000 in red, and mouse mAb to MAP-tau, NPB2-25162, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. NB300-213 antibody stains dendrites and perikarya of neurons, while NBP2-25162 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
(5 Reviews)
(75 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
INSC Antibody
NBP1-90980
Immunohistochemistry: INSC Antibody [NBP1-90980] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P
UPF2 Antibody
NBP2-20813
Western Blot: UPF2 Antibody [NBP2-20813] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: UPF2 Antibody [NBP2-20813] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: UPF2 Antibody [NBP2-20813] - C. Effects of CC on the protein levels of NMD factors UPF1, UPF2, UPF3B, SMG1, SMG5, SMG6, and SMG7 in U2OS reporter cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0204978) licensed under a CC-BY licence.
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, WB
NS1-BP Antibody
NBP1-83180
Western Blot: NS1-BP Antibody [NBP1-83180] - Analysis in human cell lines U-251MG and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Immunohistochemistry-Paraffin: NS1-BP Antibody [NBP1-83180] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Western Blot: NS1-BP Antibody [NBP1-83180] - Analysis in control (vector only transfected HEK293T lysate) and IVNS1ABP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
SHP-2/PTPN11 [p
Tyr542] Antibody [Unc ...
AF3790
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Phospho-SHP‑2 (Y542) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3790), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-SHP‑2 (Y542) at approximately 72 kDa (as indicated). The lysates were also probed for total SHP-2 with Human/Mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1894). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-SHP‑2 (Y542) at approximately 72 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse Phospho-SHP‑2 (Y542) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3790). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
(1 Publication)
Species: Hu, Mu
Applications: ICC, Simple Western, WB
Methionine
Aminopeptidase 2/META ...
NBP2-80467
Flow Cytometry: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis of Daudi cells with Methionine Aminopeptidase 2/METAP2 antibody at 1/100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Staining Methionine Aminopeptidase 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Staining Methionine Aminopeptidase 2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Staufen Antibody
NBP1-33202
Staufen Antibody
Species: Hu, Mu, Rt, Ch, RM
Applications: WB, ICC/IF, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Staufen Antibody (NBP1-33202)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-33202 Applications Species
Corbet GA, Burke JM, Bublitz GR, Parker R dsRNA-induced condensation of antiviral proteins promotes PKR activation bioRxiv Jan 1 2022 (ICC/IF, Human) ICC/IF Human
Paul S, Dansithong W, Figueroa KP et al. Staufen1 in human neurodegeneration Annals of neurology Mar 21 2021 [PMID: 33745139]
Paul S, Dansithong W, Figueroa KP et al. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun Sep 7 2018 [PMID: 30194296] (WB, Human) WB Human

Reviews for Staufen Antibody (NBP1-33202) (0)

There are no reviews for Staufen Antibody (NBP1-33202). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Staufen Antibody (NBP1-33202) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Staufen Products

Bioinformatics Tool for Staufen Antibody (NBP1-33202)

Discover related pathways, diseases and genes to Staufen Antibody (NBP1-33202). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Staufen Antibody (NBP1-33202)

Discover more about diseases related to Staufen Antibody (NBP1-33202).
 

Pathways for Staufen Antibody (NBP1-33202)

View related products by pathway.

PTMs for Staufen Antibody (NBP1-33202)

Learn more about PTMs related to Staufen Antibody (NBP1-33202).
 

Research Areas for Staufen Antibody (NBP1-33202)

Find related products by research area.

Blogs on Staufen.

Check out the latest blog posts on Staufen.
Staufen1 Overabundance and the Consequent mTOR Hyperactivity in Amyotrophic Lateral Sclerosis, Spinocerebellar Ataxia Type 2, Alzheimer’s, Parkinson’s, and Huntington’s Diseases
Jamshed Arslan, Pharm D, PhD Neurodegenerative disorders involve loss of function and, ultimately, death of neurons. Selective neuronal vulnerability has been observed in a variety of neurodegenerative diseases. Fo...  Read full blog post.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Staufen Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol STAU1
Entrez
Uniprot