| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76303-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38076-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38615-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17204-25ul | |||
![Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0011.jpg "Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33202-img0008.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0005.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Staufen stained by Staufen antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33202-img0007.jpg)
![Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit IgG-IP, Lane E:Post-IP lysate from Stau1-IP; diluted at 1:3000.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP1-33202-img0006.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Silencing of STAU1 mitigates SCA2 phenotypes. aStau1 haploinsufficiency improves abnormal motor behavior of ATXN2Q127 mice as determined by rotarod behavior at 8, 12, 16, & 20 weeks of age. ATXN2Q127;Stau1+/- mice (green) have improved rotarod performance compared with ATXN2Q127 littermates (red) starting at 12 weeks of age. Note that Stau1 haploinsufficiency (orange) by itself does not alter motor function; n = 9–15 mice per group. Values shown are mean ± SE. Significance was determined using generalized estimating equations (GEE). NS, nonsignificant, *P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P < 0.01, Student t-test. d Histograms representing the quantities & sizes of ATXN2-STAU1 co-localized granules in normal vs. SCA2 FBs. e In vivo co-localization of ATXN2 (green) with Stau1 (red) to aggregates in cerebellar PCs of 24-weeks-old ATXN2Q127 mice (white arrows). Scale bar, 30 µM. f Co-localized ATXN2 (red) & STAU1 (green) to aggregates (white arrows) in cerebellar PCs from a human SCA2 brain, that are absent in an unaffected control. Scale bar, 30 µM. g Immunoprecipitation of ATXN2 with STAU1. Non-RNase A or RNase A treated HEK-293 cell extracts expressing Flag-tagged ATXN2-(Q22 or Q108) were subjected to immunoprecipitation with Flag mAb beads & analyzed by western blotting. STAU1 shows RNA-dependent interactions with wild-type & mutant ATXN2. ATXN2 also co-immunoprecipitates with DDX6 & PABPC1, known ATXN2 interactors. Representative blots of three independent experiments are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33202_rabbit-polyclonal-staufen-antibody-31020241618942.jpg)
![Western Blot: Staufen Antibody [NBP2-20508] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP2-20508-img0001.jpg "Western Blot: Staufen Antibody [NBP2-20508] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP2-20508] - Staufen antibody detects Staufen protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Staufen stained by Staufen antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20508-img0004.jpg)
![Immunohistochemistry-Paraffin: Staufen Antibody [NBP2-20508] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunohistochemistry-Paraffin-NBP2-20508-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP2-20508] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Staufen protein stained by Staufen antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20508-img0003.jpg)
![Western Blot: Staufen Antibody [NBP1-76303] - Rat brain tissue lysate with Staufen antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-76303-img0002.jpg "Western Blot: Staufen Antibody [NBP1-76303] - Rat brain tissue lysate with Staufen antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Staufen Antibody - BSA Free [NBP1-76303] - Immunofluorescence of Staufen in Human Brain cells with Staufen antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76303_rabbit-polyclonal-staufen-antibody-271120241932615.jpg)
![Immunohistochemistry: Staufen Antibody - BSA Free [NBP1-76303] - Immunohistochemistry of Staufen in human brain tissue with Staufen antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76303_rabbit-polyclonal-staufen-antibody-2711202419541533.jpg)
![Immunohistochemistry: Staufen Antibody [NBP3-38076] - Immunohistochemistry analysis of paraffin-embedded Human esophagus using Staufen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38076_rabbit-staufen-pab-1012202411535819.jpg "Immunohistochemistry: Staufen Antibody [NBP3-38076] - Immunohistochemistry analysis of paraffin-embedded Human esophagus using Staufen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: Staufen Antibody [NBP3-38076] - Western blot analysis of various lysates using Staufen Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38076_rabbit-staufen-pab-101220241243945.jpg)
![Western Blot: Staufen Antibody (S03-3G2) [NBP3-14978] - Western blot detection of Staufen in K562, C6, 3T3 cell lysates using Staufen Rabbit mAb (1:1000 diluted). Predicted band size: 63kDa. Observed band size: 63kDa.](http://images.novusbio.com/fullsize/Staufen-Antibody-S03-3G2-Western-Blot-NBP3-14978-img0001.jpg "Western Blot: Staufen Antibody (S03-3G2) [NBP3-14978] - Western blot detection of Staufen in K562, C6, 3T3 cell lysates using Staufen Rabbit mAb (1:1000 diluted). Predicted band size: 63kDa. Observed band size: 63kDa.")
![Immunohistochemistry-Paraffin: Staufen Antibody (S03-3G2) [NBP3-14978] - Immunohistochemistry of Staufen in paraffin-embedded Human breast cancer tissue using Staufen Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Staufen-Antibody-S03-3G2-Immunohistochemistry-Paraffin-NBP3-14978-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP2-38615] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38615-img0004.jpg "Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP2-38615] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: Staufen Antibody [NBP2-38615] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunohistochemistry-Paraffin-NBP2-38615-img0003.jpg)
![Western Blot: Staufen Antibody [NBP1-57386] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-57386-img0002.jpg "Western Blot: Staufen Antibody [NBP1-57386] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: Staufen Antibody [NBP1-57386] - Human Heart Tissue, antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunohistochemistry-Paraffin-NBP1-57386-img0001.jpg)
![Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP2-22308-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Staufen Antibody [NBP2-22308] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22308 used for IP at 6 ug/mg lysate. Staufen1 was also immunoprecipitated by rabbit anti-Staufen1 antibody. For blotting immunoprecipitated Staufen1, NBP2-22308 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP2-22308-img0002.jpg)
![Western Blot: Staufen Antibody (8J5A6) [NBP3-16450] - Western blot analysis of extracts of U-87MG cells, using Staufen Rabbit mAb (NBP3-16450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Staufen-Antibody-8J5A6-Western-Blot-NBP3-16450-img0001.jpg "Western Blot: Staufen Antibody (8J5A6) [NBP3-16450] - Western blot analysis of extracts of U-87MG cells, using Staufen Rabbit mAb (NBP3-16450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Staufen Antibody (8J5A6) [NBP3-16450] - Western blot analysis of extracts of various cell lines, using Staufen Rabbit mAb (NBP3-16450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Staufen-Antibody-8J5A6-Western-Blot-NBP3-16450-img0002.jpg)
![Western Blot: Staufen Antibody [NBP3-17204] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP3-17204-img0001.jpg "Western Blot: Staufen Antibody [NBP3-17204] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Western Blot: Staufen Overexpression Lysate (Adult Normal) [NBP2-11276] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Staufen.](http://images.novusbio.com/fullsize/Staufen-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11276-img0001.jpg "Western Blot: Staufen Overexpression Lysate (Adult Normal) [NBP2-11276] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Staufen.")
![Western Blot: Staufen Overexpression Lysate (Adult Normal) [NBL1-16532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Staufen.](http://images.novusbio.com/fullsize/Staufen-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16532-img0002.jpg "Western Blot: Staufen Overexpression Lysate (Adult Normal) [NBL1-16532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Staufen.")