Rat STAT5b ELISA Kit (Colorimetric)

ELISA: Rat STAT5b ELISA Kit (Colorimetric) [NBP2-80282] - Samples were spiked with high concentrations of Rat STAT5b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat STAT5b ELISA Kit (Colorimetric) [NBP2-80282] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL

Rat STAT5b ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat STAT5B in samples. No significant cross-reactivity or interference between Rat STAT5B and analogues was observed.
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.83%
• Intra-Assay: CV% < 5.25%
• Spike Recovery: 90-111%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: STAT5B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat STAT5b ELISA Kit (Colorimetric)

• signal transducer and activator of transcription 5B
• STAT5
• STAT5b
• transcription factor STAT5B

Background

STAT5B is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for STAT5b ELISA Kit (NBP2-80282)(1)

Publication using NBP2-80282

• Elbaset MA, Mohamed BMSA, Gad SA et al. Erythropoietin mitigated thioacetamide-induced renal injury via JAK2/STAT5 and AMPK pathway Scientific reports 2023-09-11 [PMID: 37697015] (ELISA)

FAQs for STAT5b ELISA Kit (NBP2-80282). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: STAT5B