STAT5b Antibody

Western Blot: STAT5b Antibody [NB100-56324] - Analysis of Stat5b in 10 ug of Jurkat cell lysate using 1 ug/ml this antibody. Immunoreaction was detected by chemiluminescence

Product Discontinued

STAT5b Antibody Summary

• Immunogen: This polyclonal antibody was developed against a synthetic peptide corresponding to amino acids 771-787 of human Stat5b.
• Specificity: In Jurkat, a 92 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT5B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
• Control:

  Jurkat Whole Cell Lysate

Reactivity Notes

Predicted to react with Bovine, Chicken, Mouse, New World Monkey and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for STAT5b Antibody

• signal transducer and activator of transcription 5B
• STAT5
• STAT5b
• transcription factor STAT5B

Background

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. Both Stat5a and Stat5b regulate interleukin-7 induced B-cell precursor expansion. Stat5b may also act as a transcriptional inhibitor as demonstrated by inhibition of NF-kB mediated signaling. This Stat5b-mediated inhibitory effect on NF-kB signaling does not depend on Stat5b-DNA interactions but requires the carboxyl terminus of Stat5b as well as Stat5b nuclear translocation and/or accumulation, suggesting that Stat5b is competing for a nuclear factor(s) necessary for NF-kB-mediated activation of target promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT5B
• Uniprot:
  • P51692()