STAT5A Antibody (251619) [Janelia Fluor® 525]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 251619
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

STAT5A Antibody (251619) [Janelia Fluor® 525] Summary

• Immunogen: Human STAT5a synthetic peptide
  SLDSRLSPPAGLFTSARGSLS
  Accession # NP_003143
• Specificity: Detects human and mouse STAT5a. Does not cross-react with STAT5b.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT5A
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for STAT5A Antibody (251619) [Janelia Fluor® 525]

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT5A