STAT1 [p Tyr701] Antibody - BSA Free

Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825] - Shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-a (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

STAT1 [p Tyr701] Antibody - BSA Free Summary

• Description: This product was Protein A purified from monospecific antiserum
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This Protein A purified STAT1 [p Tyr701] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein. (Uniprot: Q99K94)
• Modification: p Tyr701
• Specificity: This antibody is specific for mouse STAT1 protein phosphorylated at Y701. A BLAST analysis was used to suggest cross-reactivity with STAT1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against STAT1 from human sources as 90% homology is noted. Cross-reactivity with STAT1 from other sources has not been determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with STAT1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against STAT1 from human sources as 90% homology is noted. Cross-reactivity with STAT1 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for STAT1 [p Tyr701] Antibody - BSA Free

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT1
• Uniprot:
  • NP_033309(Mouse)
  • Q99K94(Mouse)