StAR Antibody (5F9) - Azide and BSA Free

Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi ( Cat # H00006770-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 (Cat # H00006770-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: StAR Antibody (5F9) [H00006770-M01] - Detection limit for recombinant GST tagged STAR is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Mycoplasma
• Clone: 5F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

StAR Antibody (5F9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: STAR (AAH10550, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVG
• Localization: Mitochondrial
• Specificity: STAR - steroidogenic acute regulator
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22708737).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for StAR Antibody (5F9) - Azide and BSA Free

• cholesterol trafficker
• mitochondrial steroid acute regulatory protein
• StAR
• StARD1
• STARD1StAR-related lipid transfer (START) domain containing 1
• START domain containing 1
• START domain-containing protein 1
• steroid acute regulatory protein
• steroidogenic acute regulator
• steroidogenic acute regulatory protein
• steroidogenic acute regulatory protein, mitochondrial

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for StAR Antibody (H00006770-M01)(3)

Publications using H00006770-M01

• Adedara IA, Farombi EO. Chemoprotective effects of kolaviron on ethylene glycol monoethyl ether-induced pituitary-thyroid axis toxicity in male rats Andrologia 2012-06-19 [PMID: 22708737] (WB, Rat)
• Li B, Vachali P, Frederick JM, Bernstein PS. Identification of StARD3 as a Lutein-binding Protein in the Macula of the Primate Retina. Biochemistry;50(13):2541-9. 2011-04-05 [PMID: 21322544]
• Sedelaar JP, Isaacs JT. Tissue culture media supplemented with 10% fetal calf serum contains a castrate level of testosterone. Prostate 69(16):1724-9. 2009-12-01 [PMID: 19676093]

Bioinformatics

• Gene Symbol: STAR
• Entrez:
  • 6770(Human)
• OMIM:
  • 201710(Human)
• Uniprot:
  • AAH10550(Human)