STAP2 Antibody

Western Blot: STAP2 Antibody [NBP1-51904] - Analysis of Human Breast lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: STAP2 Antibody [NBP1-51904] - Staining of paraffin embedded Human Small Intestine. Antigen at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

STAP2 Antibody Summary

• Immunogen: Peptide with sequence C-LQKKLEKRRALEH corresponding to C-Terminus according to NP_060190.2, NP_001013863.1.
• Epitope: C-LQKKLEKRRALEH
• Specificity: This antibody is expected to recognize both reported isoforms (NP_060190.2; NP_001013863.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 49 kDa band observed in human breast lysates (calculated MW of 49.6 kDa band according to NP_060190.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAP2 Antibody

• BKSbrk kinase substrate
• Breast tumor kinase substrate
• BRK substrate
• FLJ20234
• signal transducing adaptor family member 2
• signal-transducing adaptor protein 2
• signal-transducing adaptor protein-2
• STAP-2

Background

This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAP2 Antibody (NBP1-51904)(1)

Publication using NBP1-51904

• Sekine Y, Ikeda O, Tsuji S et al. Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells J Immunol 2009-12-15 [PMID: 19933863]

Bioinformatics

• Gene Symbol: STAP2
• Entrez:
  • 55620(Human)
• Uniprot:
  • NP_060190.2(Human)