Stanniocalcin 2/STC-2 Overexpression Lysate (Native)

Images

 
Western Blot: Stanniocalcin 2 Overexpression Lysate (Adult Normal) [NBL1-16534] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Stanniocalcin 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Stanniocalcin 2/STC-2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200537 and based on accession number NM_003714. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens stanniocalcin 2 (STC2), mRNA.
Gene
STC2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Stanniocalcin 2/STC-2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Stanniocalcin 2/STC-2 Overexpression Lysate (Native)

  • Stanniocalcin 2
  • stanniocalcin-2
  • STC2
  • STC-2
  • STC-2Stanniocalcin-related protein
  • STC-related protein
  • STCRP

Background

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Stanniocalcin 2/STC-2 Lysate (NBL1-16534) (0)

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Bioinformatics

Gene Symbol STC2
Entrez