SESN2 Antibody (3B8) - Azide and BSA Free

Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 expression in transfected 293T cell line by SESN2 monoclonal antibody (M03), clone 3B8. Lane 1: SESN2 transfected lysatE (54.5 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SES2 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SESN2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml

Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - SESN2 expression in K-562 ( Cat # L009V1 ).

Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03] - SESN2 transfected lysate.

Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 transfected lysate using anti-SESN2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SESN2 rabbit polyclonal antibody.

Sandwich ELISA: SESN2 Antibody (3B8) [H00083667-M03] - Detection limit for recombinant GST tagged SESN2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 3B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SESN2 Antibody (3B8) - Azide and BSA Free Summary

• Immunogen: SESN2 (AAH13304.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEALMSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT
• Specificity: SESN2 - sestrin 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SESN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SESN2 Antibody (3B8) - Azide and BSA Free

• DKFZp761M0212
• DKFZp761M02121
• Hi95
• HI95sestrin-2
• SES2
• SEST2hypoxia induced gene 95
• sestrin 2

Background

This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SESN2 Antibody (H00083667-M03)(3)

Publications using H00083667-M03

• Lin LT, Liu SY, Leu JD et al. Arsenic trioxide-mediated suppression of miR-182-5p is associated with potent anti-oxidant effects through up-regulation of SESN2. Oncotarget. 2018-03-23 [PMID: 29662624]
• Li DD, Sun T, Wu XQ et al. The inhibition of autophagy sensitises colon cancer cells with wild-type p53 but not mutant p53 to topotecan treatment. PLoS One. 2012-01-01 [PMID: 23024792] (WB)
• Liu SY, Lee YJ, Lee TC. Association of platelet-derived growth factor receptor accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells. FEBS Lett. 2011-04-27 [PMID: 21536039]

Bioinformatics

• Gene Symbol: SESN2
• Entrez:
  • 83667(Human)
• OMIM:
  • 607767(Human)
• Uniprot:
  • AAH13304(Human)