Human Stanniocalcin 2/STC-2 ELISA Kit (Colorimetric)

ELISA: Human Stanniocalcin 2/STC-2 ELISA Kit (Colorimetric) [NBP2-60603]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 0.078 - 5 ng/mL (example only; lot dependent)
• Sensitivity: 0.95 ng/ml (example only; lot dependent)

Human Stanniocalcin 2/STC-2 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.078 - 5 ng/mL (example only; lot dependent)
• Sensitivity: 0.95 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.60% (example only; lot dependent)
• Intra-Assay: CV% < 5.20% (example only; lot dependent)
• Spike Recovery: 96.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: STC2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Stanniocalcin 2/STC-2 ELISA Kit (Colorimetric)

• Stanniocalcin 2
• stanniocalcin-2
• STC2
• STC-2
• STC-2Stanniocalcin-related protein
• STC-related protein
• STCRP

Background

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Stanniocalcin 2/STC-2 ELISA Kit (NBP2-60603)(1)

Publication using NBP2-60603

• P Poplawski, S Alseekh, U Jankowska, B Skupien-Ra, R Iwanicka-N, H Kossowska, A Fogtman, B Rybicka, J Bogus?awsk, A Adamiok-Os, K Hanusek, J Hanusek, M Koblowska, AR Fernie, A Piekie?ko- Coordinated reprogramming of renal cancer transcriptome, metabolome and secretome associates with immune tumor infiltration Cancer Cell International, 2023-01-05;23(1):2. 2023-01-05 [PMID: 36604669]

FAQs for Stanniocalcin 2/STC-2 ELISA Kit (NBP2-60603). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: STC2