STAG2 Antibody

Images

 
Western Blot: STAG2 Antibody [NB100-301] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-SA2 antibody used for WB ...read more
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Immunoprecipitation: STAG2 Antibody [NB100-301] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

STAG2 Antibody Summary

Immunogen
A 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded in part by exon 31 and 32 (LocusLink ID 10735).
Specificity
NB100-301 is specific for human SA2 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STAG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunoprecipitation 2 - 10 ug/mg of lysate
Application Notes
NB 100-301 may be used for Western Blot where a band at ~140 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry 1:500-1:2,000 Immunoprecipitation Not Done *The investigator should determine the optimal working dilution for a specific application.
Positive Control
HeLa Lysate (NB800-PC9)
Publications
Read Publication using
NB100-301 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STAG2 Antibody

  • bA517O1.1
  • cohesin subunit SA-2
  • DKFZp686P168
  • SA-2
  • SA2FLJ25871
  • SCC3 homolog 2
  • stromal antigen 2DKFZp781H1753

Background

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-298
Species: Hu, Xp
Applications: WB, ICC/IF, PLA
NBP2-20053
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-204
Species: Hu, Mu, Xp
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
NB100-207
Species: Hu, Mu
Applications: WB, IHC-P, IP, PLA
MAB1934
Species: Hu
Applications: WB, Flow, IP, CyTOF-ready
NB100-547
Species: Hu
Applications: WB, ICC/IF, IP, PLA
H00056937-M01
Species: Hu
Applications: WB, ELISA
H00009700-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-24906
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
29470002
Species: Ce
Applications: ELISA, ICC/IF, IHC
NBP2-52911
Species: Hu
Applications: WB, IHC, IHC-P
NB600-1522
Species: Hu, Mu
Applications: WB, GS, ICC/IF
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-87098
Species: Hu
Applications: IHC, IHC-P
NBP1-33002
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for STAG2 Antibody (NB100-301)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for STAG2 Antibody (NB100-301) (0)

There are no reviews for STAG2 Antibody (NB100-301). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STAG2 Antibody (NB100-301) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional STAG2 Products

Bioinformatics Tool for STAG2 Antibody (NB100-301)

Discover related pathways, diseases and genes to STAG2 Antibody (NB100-301). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for STAG2 Antibody (NB100-301)

Discover more about diseases related to STAG2 Antibody (NB100-301).
 

Pathways for STAG2 Antibody (NB100-301)

View related products by pathway.

PTMs for STAG2 Antibody (NB100-301)

Learn more about PTMs related to STAG2 Antibody (NB100-301).
 

Research Areas for STAG2 Antibody (NB100-301)

Find related products by research area.

Blogs on STAG2

There are no specific blogs for STAG2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our STAG2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol STAG2