Western Blot: STAG2 Antibody [NB100-301] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-SA2 antibody used for WB ...read more
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Immunoprecipitation: STAG2 Antibody [NB100-301] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 ...read more
NB 100-301 may be used for Western Blot where a band at ~140 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry 1:500-1:2,000 Immunoprecipitation Not Done *The investigator should determine the optimal working dilution for a specific application.
SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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