ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1/Ganglioside GD3 Antibody [Unconjugated]

Western blot shows lysates of Hs 294T human melanoma cell line, SK-Mel-28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1/Ganglioside GD3 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1
  Tyr49-Ser356
  Accession # Q92185
• Specificity: Detects human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ST8SIA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1/Ganglioside GD3 Antibody [Unconjugated]

• Alpha-2,8-sialyltransferase 8A
• alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
• disialoganglioside (GD3) synthase
• Ganglioside GD3 synthase
• Ganglioside GT3 synthase
• ganglioside-specific alpha-2,8-polysialyltransferase
• GD3 synthase)
• GD3S
• sialyltransferase 8 (alpha-N-acetylneuraminate: alpha-2,8-sialytransferase, GD3synthase) A
• sialyltransferase 8A (alpha-N-acetylneuraminate: alpha-2,8-sialyltransferase
• Sialyltransferase 8A
• Sialytransferase St8Sia I
• SIAT8A
• SIAT8-A
• SIAT8EC 2.4.99.8
• ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
• ST8SIA1
• ST8SiaI

Background

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues and are particularly prevalent on neuronal cells (1). Ganglioside GD3 is involved in cell adhesion and the growth of cultured malignant cells (2). ST8SIA1 is a sialyltransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 (NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) to produce GD3 (NeuNAc alpha 2‑8NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) and GT3 (NeuNAc alpha 2‑8NeuNAc alpha 2‑8NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) in a successive manner (3); therefore the enzyme has both GD3 and GT3 synthase activity (4). ST8SIA1 is mainly expressed in adult and fetal brain, and its expression is enhanced in melanoma cell lines (3, 4, 5). Like most known glycosyltransferases, ST8SIA1 is predicted as a type II transmembrane protein with a short N‑terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of the Golgi apparatus. However, recently GD3 synthase activity was demonstrated at the surface of epithelial and melanoma cells, suggesting glycosphingolipid synthesis may occur at the cell membrane (6). Recombinant ST8SIA1 also showed activity on fetuin from fetal calf serum, when measured using a phosphatase-coupled method (7).

1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
2. Cheresh, D.A. et al. (1986) J. Cell. Biol. 102:688.
3. Nakayama, J. et al. (1986) J. Biol. Chem. 271:3684.
4. Nara, K. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:7952.
5. Haraguchi, M. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:10455.
6. Crespo, P.M. et al. (2010) J. Biol. Chem. 285:29179.
7. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1/Ganglioside GD3 Antibody (AF6716)(3)

Publications using AF6716

• Jing Wang, Quanguang Zhang, Yujiao Lu, Yan Dong, Krishnan M. Dhandapani, Darrell W. Brann et al. Ganglioside GD3 is up‐regulated in microglia and regulates phagocytosis following global cerebral ischemia Journal of Neurochemistry 8/1/2021 [PMID: 34133773]
• A Das, R Barrientos, T Shiota, V Madigan, I Misumi, KL McKnight, L Sun, Z Li, RM Meganck, Y Li, E Kaluzna, A Asokan, JK Whitmire, M Kapustina, Q Zhang, SM Lemon Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus Nat Microbiol, 2020-05-25;0(0):. 2020-05-25 [PMID: 32451473] (Western Blot, Human)
• TR Karhadkar, D Pilling, N Cox, RH Gomer Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model Sci Rep, 2017-11-08;7(1):15069. 2017-11-08 [PMID: 29118338] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: ST8SIA1
• Uniprot:
  • Q92185()