Ceramide Kinase Antibody - BSA Free

Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.

Immunocytochemistry/Immunofluorescence: Ceramide Kinase Antibody [NB100-2911] - Ceramide Kinase antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.82 mg/ml

Ceramide Kinase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ceramide Kinase Antibody - BSA Free (NB100-2911) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-Ceramide Kinase Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal portion of human Ceramide Kinase (between residues 50-150). [Swiss-Prot# Q8TCT0]
• Localization: Cytoplasmic and membraneous, cytoplasmic vesicles
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Western Blot 2 ug/ml
• Application Notes: This Ceramide Kinase antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is seen at ~65 kDa representing the Ceramide Kinase protein, and in Immunocytochemistry/Immunofluorescence vesicular cytoplasmic staining was observed in A431 cells.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Ceramide Kinase Peptide (NB100-2911PEP)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.82 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ceramide Kinase Antibody - BSA Free

• Ceramide Kinase
• CERK
• dA59H18.2
• DKFZp434E0211
• EC 2.7.1.138
• FLJ21430
• FLJ23239
• hCERK
• hCERKLipid kinase 4
• KIAA1646
• KIAA1646dA59H18.3
• Lipid kinase 4
• lipid kinase LK4
• LK4
• LK4Acylsphingosine kinase
• MGC131878

Background

Ceramide kinase (CERK) belongs to diacylglycerol kinase family of lipid kinases and it catalyzes specifically the phosphorylation of ceramide to generate ceramide 1-phosphate (C1P). CERK acts primarily on natural as well as analog ceramides such as C6, C8, C16 ceramides, and C8-dihydroceramide, to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids including sphingosines. Localized in the cytoplasm and membrane as peripheral membrane protein, CERK is the only enzyme known to produce C1P, a signaling molecule which shows positive effect on cell proliferation or survival and also regulates cytosolic phospholipase A2 (cPLA2) activity . CERK/C1P signaling is implicated in mast cell activation, eicosanoid signaling, apoptosis, cellular immune response/inflammation, emotional behavior, angiogenesis, phagolysosome formation etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ceramide Kinase Antibody (NB100-2911)(3)

Publications using NB100-2911

• Tian L, Zhao C, Yan Y et al. Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics Journal of advanced research 2023-07-20 [PMID: 37479181] (WB, Mouse)
• Bhadwal P, Randhawa V, Vaiphei K et al. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance Scientific reports 2022-10-29 [PMID: 36309544] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Doria M Luisa, Ribeiro Ana S, Wang Jun et al. Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival. FASEB J. 2014-06-26 [PMID: 24970396] (WB, Mouse)

Bioinformatics

• Gene Symbol: CERK